Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Modulation on immunogenicity by HLA-B27 subtype polymorphism.

D Jaraquemada1, B Galocha, P Aparicio

  • 1Department of Immunology, Fundación Jiménez Díaz, Madrid, Spain.

European Journal of Immunology
|December 1, 1988
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

LUDICAS: sexual dysfunction in patients with lung cancer, a multicenter cross-sectional study.

ESMO open·2025
Same author

Dietary and competition effects on life history attributes of Chrysomya megacephala and Lucilia sericata (Diptera: Calliphoridae) in south-west Europe.

International journal of legal medicine·2025
Same author

Development and clinical validation of real-time artificial intelligence diagnostic companion for fetal ultrasound examination.

Ultrasound in obstetrics & gynecology : the official journal of the International Society of Ultrasound in Obstetrics and Gynecology·2023
Same author

Up to 300 K lasing with GeSn-On-Insulator microdisk resonators.

Optics express·2022
Same author

Assessment of self-efficacy for caregiving in oncology: Italian validation of the caregiver inventory (CGI-I).

BMC palliative care·2021
Same author

KRAS p.G12C mutation occurs in 1% of EGFR-mutated advanced non-small-cell lung cancer patients progressing on a first-line treatment with a tyrosine kinase inhibitor.

ESMO open·2021
Same journal

Fast Generation of F(Ab')<sub>2</sub> Fragments From Human IgG Using Fc-Fused IgG-Degrading Enzyme.

European journal of immunology·2026
Same journal

Generation of Regulatory T Cells Against Islet Neoantigen.

European journal of immunology·2026
Same journal

Complement Inhibition in the Clinic: Are We Doing Enough to Protect Patients From Infection?

European journal of immunology·2026
Same journal

Special Issue: yEFIS 3rd Symposium.

European journal of immunology·2026
Same journal

CRISPR/Cas9-Mediated Gene Knockout Reveals a Nonredundant Role for p16<sup>INK4A</sup> in Controlling TCR-Dependent and Independent CD8 T Cell Expansion.

European journal of immunology·2026
Same journal

Induction of Humoral and Cellular Immunity After SARS-CoV-2 JN.1 Vaccination in Individuals With and Without Prior Infection.

European journal of immunology·2026
See all related articles

Cytolytic T lymphocytes (CTL) responses to HLA-B27 subtypes vary significantly. Minor amino acid changes in B*2704 reduce its immunogenicity compared to B*2705 in specific individuals, impacting CTL recognition.

Area of Science:

  • Immunology
  • Molecular Biology
  • Genetics

Background:

  • Human Leukocyte Antigen (HLA) class I molecules play a crucial role in immune responses, particularly in T cell recognition.
  • HLA-B27 is associated with various autoimmune diseases, and understanding its subtypes' immunogenicity is critical for immune research.
  • Previous studies indicated that CTL clones recognizing B*2705 do not cross-react with B*2704, suggesting specific immunodominant determinants.

Purpose of the Study:

  • To investigate the immunogenicity of HLA-B27 subtypes, specifically comparing B*2705 and B*2704 in allogeneic mixed lymphocyte cultures.
  • To determine if amino acid differences at positions 77 and 152 in B*2704 alter T cell receptor recognition compared to B*2705.
  • To characterize the specificity of CTL responses against different HLA-B27 subtypes.

Related Experiment Videos

Main Methods:

  • Primary mixed lymphocyte cultures were established using cells from an HLA-B27- individual stimulated with B*2705+ or B*2704+ lymphoblastoid cell lines.
  • Cytolytic T lymphocytes (CTL) were cloned by limiting dilution and screened for anti-B27 alloreactivity.
  • CTL clones were tested for cross-reactivity against various HLA-B27 subtypes, including B*2703.

Main Results:

  • CTL clones generated against B*2705 were primarily directed against the B*2705 antigen.
  • No anti-B27 CTL clones were found when stimulated with B*2704, despite a robust cytotoxic response from T cell clones.
  • A single CTL clone (64.8P) reactive to B*2704 cross-reacted with most B27 subtypes except B*2703, indicating residues 77 and 152 are not critical for its recognition.

Conclusions:

  • Amino acid differences at positions 77 and 152 in B*2704 significantly reduce its immunogenicity for certain individuals compared to B*2705.
  • The immunodominant determinant for B*2705 recognition is likely influenced by residues 77 and/or 152.
  • The findings highlight the subtle yet significant impact of minor amino acid variations in HLA molecules on T cell-mediated immune responses.