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Related Concept Videos

Ion-Exchange Chromatography01:09

Ion-Exchange Chromatography

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Ion-exchange chromatography, or IEC, is a technique for separating ions based on their affinity for the stationary phase. The stationary phase is a cross-linked polymer resin with covalently attached ionic functional groups. The functional groups can be either positively charged (cation exchangers) or negatively charged (anion exchangers). A cation exchanger consists of a polymeric anion and active cations, while an anion exchanger is a polymeric cation with active anions. The choice of...
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Types Of Column Chromatography01:29

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The stability and compatibility of column material with samples are crucial for efficient purification in chromatographic techniques. Various operating parameters such as pH, temperature, or solvent affect the packing of the column material, thereby determining the purification efficiency. The choice of column material also plays an essential role in deciding the operating parameters and can be modified based on the proteins that need to be purified.
Gel Filtration Chromatography
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Principles Of Column Chromatography01:13

Principles Of Column Chromatography

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The chromatography technique was first invented in 1901 by Michael S. Tswett, a Russian botanist, to separate plant pigments using organic solvents. Further, in 1941, Archer John Porter Martin and R. L. M. Synge modified the technique by packing silica gel into a column. A mixture of amino acids was then separated on the packed column using chloroform and water mixture as the mobile phase. This was the first report on column chromatography. At present, column chromatography is a widely used...
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In High-Performance Liquid Chromatography (HPLC), the elution process is critical to the separation of analytes and the quality of chromatographic results. Elution describes how compounds move through the column and separate based on their interactions with the mobile and stationary phases. This process determines the resolution, peak shape, and retention times in the chromatogram, which are essential for identifying and quantifying components in complex mixtures. Understanding the elution...
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High-Performance Liquid Chromatography: Introduction01:11

High-Performance Liquid Chromatography: Introduction

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High-performance liquid chromatography(HPLC), formerly referred to as High-pressure liquid chromatography, is a powerful technique used to separate, identify, and quantify components in complex mixtures. The term "high pressure" refers to using high pressure to push the liquid mobile phase through the tightly packed columns.
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Related Experiment Video

Updated: May 2, 2026

Cellular Membrane Affinity Chromatography Columns to Identify Specialized Plant Metabolites Interacting with Immobilized Tropomyosin Kinase Receptor B
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High-throughput process development: II. Membrane chromatography.

Anurag S Rathore1, Sampath Muthukumar

  • 1Department of Chemical Engineering, Indian Institute of Technology, Hauz Khas, New Delhi, 110016, India, asrathore@biotechcmz.com.

Methods in Molecular Biology (Clifton, N.J.)
|March 21, 2014
PubMed
Summary
This summary is machine-generated.

This protocol details high-throughput process development for membrane chromatography, offering a faster alternative to traditional methods for biopharmaceutical purification. It ensures data accuracy comparable to lab-scale experiments, valuable for the biopharma industry.

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Area of Science:

  • Biochemistry
  • Chemical Engineering
  • Process Development

Background:

  • Membrane chromatography offers advantages over conventional column chromatography, including reduced pressure drop and faster solute transport.
  • It is increasingly preferred for biopharmaceutical polishing, especially monoclonal antibodies, due to time and resource constraints in the industry.

Purpose of the Study:

  • To describe a protocol for high-throughput process development (HTPD) of membrane chromatography.
  • To present a method for statistical analysis of data generated from HTPD membrane chromatography.

Main Methods:

  • Utilized a commercially available 96-well format device (AcroPrep™ Advance filter plate with Mustang S membrane).
  • Addressed challenges and solutions for performing HTPD experiments.
  • Applied statistical analysis to HTPD data.

Main Results:

  • Demonstrated the protocol's utility through a case study on Granulocyte Colony Stimulating Factor (GCSF) ion exchange chromatography.
  • Achieved significant agreement between HTPD data and traditional lab-scale data (regression coefficient 0.99).

Conclusions:

  • The developed protocol is valuable for high-throughput process development of membrane chromatography.
  • It generates data representative of traditional lab-scale methods, aiding biopharmaceutical development.