Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

IR Spectrometers01:25

IR Spectrometers

There are two main infrared (IR) spectrophotometers: dispersive IR spectrometers and Fourier transform infrared (FTIR) spectrometers. In a dispersive IR spectrometer, a beam of infrared radiation produced by a hot wire is divided into two parallel equal-intensity beams using mirrors. One beam passes through the sample, while another is a reference beam. The beams then move through the monochromator, which separates the radiations into a continuous spectrum of different frequencies. The...
IR Frequency Region: Fingerprint Region01:03

IR Frequency Region: Fingerprint Region

IR spectra are divided into two main regions: the diagnostic region and the fingerprint region. The diagnostic region of the spectrum lies above 1500 cm−1. The absorptions resulting from single-bond vibrations of the N–H, C–H, and O–H stretch at higher wavenumbers and appear on the left side of the spectrum. The stretching absorptions of the C≡C and C≡N occur between 2100–2300 cm−1. In contrast, those arising from stretching absorptions of the C=O, C=N, and C=C occur between 1600–1850 cm−1.
The...
Applications of IR Spectroscopy: Overview01:11

Applications of IR Spectroscopy: Overview

The non-destructive nature and ability to provide valuable chemical information make IR spectroscopy a versatile technique with broad applications in various scientific and industrial fields. IR spectroscopy is commonly used to identify and characterize organic and inorganic compounds. It provides information about the functional groups present in a molecule and the bonding between atoms. This helps in the structural elucidation of compounds during organic synthesis, pharmaceutical research,...
Rapid Identification of Pathogens01:25

Rapid Identification of Pathogens

MALDI-TOF MS has transformed clinical microbiology by offering a rapid and reliable method for pathogen identification. The traditional approach to microbial identification typically involves time-consuming culture techniques and biochemical tests, which can delay the initiation of appropriate antimicrobial therapy. MALDI-TOF MS avoids these delays by using characteristic ribosomal protein mass patterns of microbial cells, enabling accurate species-level identification within minutes.Principle...
Infrared (IR) Spectroscopy: Overview01:09

Infrared (IR) Spectroscopy: Overview

When electromagnetic radiation passes through a material, atoms or molecules transition from a lower to a higher energy state by absorbing radiation corresponding to the energy difference between the two states. The absorption of infrared (IR) radiation causes transitions between vibrational energy levels in a molecule. Therefore, IR spectroscopy is a useful analytical tool for determining the molecular structure of molecules.
Different compounds display unique properties due to their...
IR Spectrum01:19

IR Spectrum

When infrared (IR) radiation passes through a molecule, the bonds stretch or bend by absorbing the radiation. This absorption creates the molecule's absorption spectrum, which is the plot of its percentage transmittance versus wavenumber.
Transmittance is defined as the ratio of the radiant power passing through a sample to that from the radiation's source. Multiplying the transmittance by 100 gives the percent transmittance (%T), which varies between 100% (no absorption) and 0% (complete...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Osmolyte-Based Formulations for Enhanced Thermal Stability of mRNA Drug Substance: A Systematic Screening and Optimization Study.

Pharmaceutical research·2026
Same author

Structural Elucidation of Fc- and Fab-Associated <i>N</i>-Glycans in Cetuximab Using Protein A-Assisted Domain-Resolved Glycan Profiling Using Mass Spectrometry.

Journal of the American Society for Mass Spectrometry·2026
Same author

A Multitechnique Spectroscopy Platform for Monitoring Heterogeneities in the Higher-Order Structure of mAb Therapeutics.

Molecular pharmaceutics·2026
Same author

A Prediction Framework for Quantification of Milk Adulterants Using a NIR-Coupled Boosting Algorithm.

Journal of food science·2026
Same author

Best Practices for Performing Analytical and Functional Biosimilarity Assessment of Recombinant Monoclonal Antibody Biosimilars.

The AAPS journal·2026
Same author

Continuous Processing for Manufacturing of Antibody-Drug Conjugates (ADCs).

Bioconjugate chemistry·2026

Related Experiment Video

Updated: Jun 11, 2026

High-definition Fourier Transform Infrared (FT-IR) Spectroscopic Imaging of Human Tissue Sections towards Improving Pathology
11:05

High-definition Fourier Transform Infrared (FT-IR) Spectroscopic Imaging of Human Tissue Sections towards Improving Pathology

Published on: January 21, 2015

Rapid Identification of Counterfeit Biopharmaceuticals using Portable Fourier Transform Infrared Spectroscopy.

Vineela Peruri1, Rishika Trivedi1, Drashti Trivedi1

  • 1Department of Chemical Engineering, Indian Institute of Technology, New Delhi, India.

AAPS Pharmscitech
|June 9, 2026
PubMed
Summary

Fourier-Transform Infrared (FTIR) spectroscopy offers a rapid method for detecting counterfeit biopharmaceuticals. This technique, combined with machine learning, ensures product integrity and quality assurance in manufacturing and distribution.

Keywords:
FTIRbiosimilarscounterfeit drugsmonoclonal antibodies

More Related Videos

Atomic Force Microscopy Combined with Infrared Spectroscopy as a Tool to Probe Single Bacterium Chemistry
08:51

Atomic Force Microscopy Combined with Infrared Spectroscopy as a Tool to Probe Single Bacterium Chemistry

Published on: September 15, 2020

Optical Photothermal Infrared-Fluorescence In Situ Hybridization (OPTIR-FISH)
04:07

Optical Photothermal Infrared-Fluorescence In Situ Hybridization (OPTIR-FISH)

Published on: February 23, 2024

Related Experiment Videos

Last Updated: Jun 11, 2026

High-definition Fourier Transform Infrared (FT-IR) Spectroscopic Imaging of Human Tissue Sections towards Improving Pathology
11:05

High-definition Fourier Transform Infrared (FT-IR) Spectroscopic Imaging of Human Tissue Sections towards Improving Pathology

Published on: January 21, 2015

Atomic Force Microscopy Combined with Infrared Spectroscopy as a Tool to Probe Single Bacterium Chemistry
08:51

Atomic Force Microscopy Combined with Infrared Spectroscopy as a Tool to Probe Single Bacterium Chemistry

Published on: September 15, 2020

Optical Photothermal Infrared-Fluorescence In Situ Hybridization (OPTIR-FISH)
04:07

Optical Photothermal Infrared-Fluorescence In Situ Hybridization (OPTIR-FISH)

Published on: February 23, 2024

Area of Science:

  • Analytical Chemistry
  • Spectroscopy
  • Biopharmaceutical Analysis

Background:

  • Biopharmaceuticals are costly due to complex manufacturing, characterization, and storage requirements.
  • High costs make biopharmaceuticals targets for counterfeiters, posing significant public health risks.
  • Identifying counterfeit biopharmaceuticals is challenging due to product complexity and the need for specialized analytical expertise, particularly in low-income regions.

Purpose of the Study:

  • To develop a simple, rapid method for identifying counterfeit biopharmaceuticals using Fourier-Transform Infrared (FTIR) spectroscopy.
  • To assess the utility of FTIR spectroscopy combined with statistical and machine learning approaches for quality assurance.
  • To evaluate the method's effectiveness in detecting counterfeit, biosimilar, and compromised potency biopharmaceutical products.

Main Methods:

  • Utilized FTIR spectroscopy to analyze a dataset of 43 monoclonal antibodies (mAbs) and 42 non-mAb products.
  • Applied pre-processing techniques including noise reduction and baseline correction.
  • Employed Principal Component Analysis (PCA) and K-means clustering for data analysis and counterfeit detection, with validation via RP-HPLC.

Main Results:

  • The FTIR method successfully identified deviations in both mAb and non-mAb therapeutics.
  • Outlier biosimilars in PCA score plots were flagged as potential counterfeits.
  • Diluted mAbs (70% concentration) were effectively distinguished using PCA and K-means clustering.
  • Counterfeit detection was validated using RP-HPLC analysis.

Conclusions:

  • FTIR spectroscopy provides a rapid, label-free, and non-destructive solution for biopharmaceutical quality assurance.
  • The integrated FTIR, statistical, and machine learning approach enables efficient real-world counterfeit detection.
  • This methodology enhances the integrity of biopharmaceutical manufacturing and distribution supply chains.