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Modification of a trypsin-detergent method for DNA flow cytometry of human epidermis
D B Stough1, E R Burns, S B Mallory
1Department of Dermatology, University of Arkansas for Medical Sciences, Little Rock 72205.
Cytometry
|January 1, 1989
Abstract:
An existing technique (Vindelov et al. Cytometry 3:323, 1983) has been modified for DNA flow cytometry of human epidermis obtained from 2 to 3 mm punch biopsies. By varying the length of time of digestion of the epidermal disc by trypsin from 5 to 70 min a controlled release of keratinocytes occurred beginning with the stratum basale and proceeding toward, but not including, the superficial layer of the epidermis, the stratum corneum.