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Author Spotlight: High-Sensitivity Tissue Factor Activity Assay for Plasma Diagnosis
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Procoagulant tissue factor activity on microparticles is associated with disease severity and bacteremia in febrile

F J Sherida H Woei-A-Jin1, Willize E van der Starre2, Margot E T Tesselaar3

  • 1Department of Clinical Oncology, Leiden University Medical Center, 2300 RC Leiden, The Netherlands; Einthoven Laboratory for Experimental Vascular Medicine, Leiden University Medical Center, 2300 RC Leiden, The Netherlands.

Thrombosis Research
|March 25, 2014
PubMed
Abstract

Insights

Procoagulant tissue factor (TF) microparticles are elevated in patients with E. coli urinary tract infections, correlating with disease severity and bacteremia. These findings suggest TF microparticles contribute to the prothrombotic state in sepsis.

Area of Science:

  • Biochemistry
  • Immunology
  • Infectious Diseases

Background:

  • Tissue factor (TF) initiates coagulation and is implicated in sepsis pathogenesis.
  • Microparticles expressing TF (MP-TF) are released during inflammation and coagulation.
  • The role of MP-TF in E. coli urinary tract infections (UTI) requires investigation.

Purpose of the Study:

  • To investigate the association between MP-TF release and procoagulant/inflammatory changes in patients with febrile E. coli UTI.
  • To correlate MP-TF activity with clinical disease severity and bacteremia.

Main Methods:

  • A multicenter cohort study of 215 patients with febrile E. coli UTI.
  • Measurement of plasma MP-TF activity, TAT, sE-selectin, sVCAM-1, procalcitonin, and monocyte count.
  • Assessment of clinical disease severity using APACHE II scores.

Main Results:

  • Plasma MP-TF activity correlated with APACHE II scores and weakly with inflammatory markers.
  • MP-TF activity was significantly higher in patients than controls, and highest in bacteremic patients.
  • MP-TF activity and TAT levels decreased after antibiotic treatment.

Conclusions:

  • Elevated MP-TF activity is linked to disease severity and bacteremia in E. coli UTI.
  • MP-TF may contribute to the prothrombotic state observed in gram-negative sepsis.
Keywords:
CoagulationDisease severityInflammationMicroparticlesSepsisTissue factor

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