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Cell sedimentation with gravity activation
G Czerlinski1, R Goldman-Leikin, D Reid
1Northwestern University Medical School, Chicago, IL 60611.
Cell Biophysics
|December 1, 1988
Summary
Murine monoclonal antibody T101 was attached to heavy alloy particles and then to T-cell leukemia cells. Researchers calculated approximately 33 particles per cell, with a surface saturation of 144 particles/cell.
Area of Science:
- Biotechnology
- Materials Science
- Oncology
Background:
- Murine monoclonal antibody T101 targets T-cells.
- Heavy alloy particles (LaMn2Ge2) offer unique physical properties.
- T-cell leukemia cell line RPMI 8402 (T8402) is a model for leukemia research.
Purpose of the Study:
- To conjugate T101 antibody-bound particles to T-cell leukemia cells.
- To quantify the number of particles attached to each cell.
- To determine the surface saturation capacity of the cells for these particles.
Main Methods:
- Coupling of T101 antibody to polymer-coated LaMn2Ge2 particles.
- Attachment of antibody-particle conjugates to T8402 cells.
- Measurement of sedimentation velocities for cells, particles, and conjugates.
- Calculation of mean particle radii and number of particles per cell.
Main Results:
- A mean of 33 T101-bound particles were calculated to attach to each T8402 cell.
- Surface saturation was estimated at 144 particles per cell.
- The study considered particle and cell radii ranging from 0.01 to 1 micron and 4 to 10 microns, respectively.
Conclusions:
- The study successfully quantified particle attachment to leukemia cells.
- Findings provide insights into particle-cell conjugation for potential therapeutic or diagnostic applications.
- Theoretical calculations support experimental observations on particle loading and saturation.