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Updated: May 1, 2026
Combining Peripheral Nerve Grafting and Matrix Modulation to Repair the Injured Rat Spinal Cord
Published on: November 20, 2009
The mesenchymal stem cell-derived microvesicles enhance sciatic nerve regeneration in rat: a novel approach in
Abbas Raisi1, Saeed Azizi, Nowruz Delirezh
1From the Departments of Clinical Sciences (A.R., S.A.), and Pathobiology (A.A.F.), Faculty of Veterinary Medicine, and Department of Cellular and Molecular Biotechnology (N.D.), Institute of Biotechnology, Urmia University; and Neurophysiology Research Center (B.H.), Urmia University of Medical Sciences, Urmia, Iran; and Department of Veterinary Pathology (K.A.), Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, Saskatchewan, Canada.
Background:
The accomplishment for desired functional peripheral nerve regeneration is still challenging despite various materials and methods. The effects of local application of omental adipose mesenchymal stromal cell-derived microvesicles (MVs) on peripheral nerve regeneration were studied using a rat sciatic nerve transection model.
Methods:
A 10-mm gap of sciatic nerve was bridged with a chitosan conduit. The rats were divided into five experimental groups randomly as follows: cultured undifferentiated omental adipose-derived stromal cells, rest mesenchymal stem cell-derived MVs (c-MVs), anti-inflammatory mesenchymal stem cell-derived MVs (anti-MVs), proinflammatory mesenchymal stem cell-derived MVs (pro-MVs), and negative control (Chit).
Results:
The functional assessment of nerve regeneration (walking track analyses), electrophysiologic measurements, muscle mass measurements, as well as histomorphometrical and immunohistochemical indices showed drastic improvement in nerve regeneration in c-MVs and anti-MVs animals compared with pro-MVs animals (p < 0.05).
Conclusion:
The anti-inflammatory stem cell-derived MVs can be used as an alternative for the improvement of rat sciatic nerve regeneration.
