[A method of immunoenzyme analysis for determining human histocompatibility antigens]

Enzyme immunoassay (EIA) has been applied to measurements of human histocompatibility (HLA) antigens (antibodies). The optimal concentration of the antigen immobilized on solid phase and the optimal dilutions of HLA sera have been found. The assays have been performed with the peripheral blood lymphocytes and platelets from donors with a known HLA phenotype. Horseradish peroxidase (Sigma, Rz-2.75) has been employed as the enzymic label, polystyrene tubes manufactured by LKB (Sweden) have been used as solid-phase carriers. The results evidence the possibility of applying EIA to the determination of HLA antigens.