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Updated: May 1, 2026

Super-Resolution Imaging and Shared Management: A Protocol for Confocal Microscopy with Multiplex Detection
Published on: February 24, 2026
Introduction to super-resolution microscopy
Masahito Yamanaka1, Nicholas I Smith1, Katsumasa Fujita2
1Department of Applied Physics Immunology Frontier Research Center, Osaka University, Suita, Osaka, Japan.
Abstract:
In this review, we introduce the principles of spatial resolution improvement in super-resolution microscopies that were recently developed. These super-resolution techniques utilize the interaction of light and fluorescent probes in order to break the diffraction barrier that limits spatial resolution. The imaging property of each super-resolution technique is also compared with the corresponding conventional one. Typical applications of the super-resolution techniques in biological research are also introduced.
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