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Quantitative Optical Microscopy: Measurement of Cellular Biophysical Features with a Standard Optical Microscope
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Quantitative phase microscopy with off-axis optical coherence tomography.

Matthew T Rinehart, Volker Jaedicke, Adam Wax

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    |April 2, 2014
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    Summary
    This summary is machine-generated.

    We developed quantitative phase imaging using spectral domain optical coherence tomography. This method uses an off-axis reference beam to eliminate artifacts and provide depth-resolved phase measurements.

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    Area of Science:

    • Optical Coherence Tomography
    • Phase Imaging
    • Metrology

    Background:

    • Spectral domain optical coherence tomography (SD-OCT) is a powerful imaging technique.
    • Quantitative phase imaging (QPI) provides contrast for transparent samples.
    • Complex conjugate artifact is a limitation in spectral interferometry.

    Purpose of the Study:

    • To develop a QPI modality within SD-OCT.
    • To enable depth-resolved quantitative phase measurements.
    • To eliminate the complex conjugate artifact.

    Main Methods:

    • Utilized an off-axis reference beam in SD-OCT.
    • Tilted the reference beam relative to the sample beam to generate spatial fringes.
    • Employed a parallel spectral domain detection scheme to separate the interference signal.
    • Obtained the complex sample field for QPI.

    Main Results:

    • Successfully implemented QPI within SD-OCT.
    • Achieved depth-resolved quantitative phase measurements.
    • Demonstrated elimination of the complex conjugate artifact.
    • Validated the approach using technical samples.

    Conclusions:

    • The developed off-axis reference beam modality enables artifact-free QPI in SD-OCT.
    • This technique enhances the capabilities of SD-OCT for quantitative measurements.
    • The method offers a robust solution for overcoming limitations in spectral interferometry.