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Cryo-electron Microscopy

Cryo-electron Microscopy

Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...

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Related Experiment Video

Updated: May 1, 2026

A Robust Single-Particle Cryo-Electron Microscopy cryo-EM Processing Workflow with cryoSPARC, RELION, and Scipion

A Robust Single-Particle Cryo-Electron Microscopy cryo-EM Processing Workflow with cryoSPARC, RELION, and Scipion

Published on: January 31, 2022

Capturing RNA-dependent pathways for cryo-EM analysis.

Justin R Tanner¹, Katherine Degen², Brian L Gilmore¹

¹Virginia Tech Carilion Research Institute, Roanoke, VA, 24016, USA.

Computational and Structural Biotechnology Journal

|April 2, 2014

Summary

This summary is machine-generated.

This study introduces a new molecular imaging platform using cryo-electron microscopy to visualize multiple cellular pathway components together. This advance allows for studying RNA-dependent pathways in their functional context.

Keywords:

Affinity Capture technology Lipid monolayer Protein synthesis Transcription

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Cryosectioning of Contiguous Regions of a Single Mouse Skeletal Muscle for Gene Expression and Histological Analyses

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Cryosectioning of Contiguous Regions of a Single Mouse Skeletal Muscle for Gene Expression and Histological Analyses

Cryosectioning of Contiguous Regions of a Single Mouse Skeletal Muscle for Gene Expression and Histological Analyses

Published on: December 12, 2016

Area of Science:

Structural Biology
Molecular Imaging
Biochemistry

Background:

Cryo-electron microscopy (EM) typically studies isolated macromolecular assemblies.
Existing methods lack the ability to view cellular components within their native context.
Visualizing dynamic biological processes in situ remains a significant challenge.

Purpose of the Study:

To develop a novel molecular imaging platform for visualizing multiple components of cellular pathways simultaneously.
To capture and view these components within a functionally relevant framework.
To advance the study of RNA-dependent biological pathways.

Main Methods:

Utilized modified Affinity Grid surfaces for targeted recruitment of protein assemblies.
Employed Affinity Capture technology in conjunction with single particle electron microscopy.
Developed an approach using the bacterial protein synthesis machinery as a model system.

Main Results:

Successfully recruited multiple protein assemblies bound to nascent mRNA strands onto the Affinity Grid.
Demonstrated the capability to visualize these assemblies in a functionally relevant context.
Established a new method for observing RNA-dependent pathways.

Conclusions:

The developed platform enables visualization of multiple cellular pathway components in situ.
This technique provides a novel way to study RNA-dependent pathways.
Advances in cryo-electron microscopy and affinity capture offer new insights into complex biological systems.