Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

9.1K
Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
9.1K
Phase Contrast and Differential Interference Contrast Microscopy01:26

Phase Contrast and Differential Interference Contrast Microscopy

9.4K
Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
9.4K
Three-Dimensional Microscopy in Microbiology01:28

Three-Dimensional Microscopy in Microbiology

907
Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...
907

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Efficacy and safety of 24 antibiotics for group A streptococcal pharyngitis: a network meta-analysis of 64 randomized controlled trials.

Frontiers in public health·2026
Same author

Threshold effect of the METS-VF index on hyperuricemia in adults with hypertension: a cross-sectional study.

BMC endocrine disorders·2026
Same author

Digging deeper into NINJ1: its multifaceted role in central nervous system diseases.

Frontiers in immunology·2026
Same author

Elucidating the protective role of quercetin against lipopolysaccharide-induced necroptosis in broiler thymus: insights from Nrf2/PERK signaling based on network pharmacology and experimental validation.

Redox report : communications in free radical research·2026
Same author

Short-exposure annular LED array-based digital refocusing microscopy via multiplexed illumination.

Applied optics·2026
Same author

Mitotic spindle-organizing protein 2A (MZT2A) promotes cisplatin resistance through NEMO ubiquitination and NF-κB activation in lung adenocarcinoma.

The Journal of biological chemistry·2026

Related Experiment Video

Updated: May 1, 2026

Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization
10:28

Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization

Published on: July 5, 2016

9.5K

Dynamic imaging through turbid media based on digital holography.

Shiping Li, Jingang Zhong

    Journal of the Optical Society of America. A, Optics, Image Science, and Vision
    |April 3, 2014
    PubMed
    Summary

    This study presents a digital holography method for dynamic imaging through turbid media. The technique successfully captures moving objects obscured by scattering, offering a safer alternative to X-rays.

    Area of Science:

    • Optics and Photonics
    • Biomedical Imaging
    • Holography

    Background:

    • Imaging through turbid media using visible or IR light is challenging, particularly for dynamic scenes.
    • Current methods often involve harmful X-rays or lack dynamic imaging capabilities.
    • Scattering in turbid media significantly degrades image quality and obscures objects.

    Purpose of the Study:

    • To develop and demonstrate a digital holography method for dynamic imaging through turbid media.
    • To overcome limitations of focusing and low contrast in scattering media.
    • To provide a safer, non-ionizing imaging alternative.

    Main Methods:

    • Utilized digital holography with a continuous-wave (cw) laser to match coherence lengths.
    • Implemented an autofocus technology to address focusing difficulties in scattering media.

    More Related Videos

    Digital Inline Holographic Microscopy DIHM of Weakly-scattering Subjects
    10:16

    Digital Inline Holographic Microscopy DIHM of Weakly-scattering Subjects

    Published on: February 8, 2014

    11.5K
    Quantifying Microorganisms at Low Concentrations Using Digital Holographic Microscopy DHM
    07:27

    Quantifying Microorganisms at Low Concentrations Using Digital Holographic Microscopy DHM

    Published on: November 1, 2017

    9.8K

    Related Experiment Videos

    Last Updated: May 1, 2026

    Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization
    10:28

    Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization

    Published on: July 5, 2016

    9.5K
    Digital Inline Holographic Microscopy DIHM of Weakly-scattering Subjects
    10:16

    Digital Inline Holographic Microscopy DIHM of Weakly-scattering Subjects

    Published on: February 8, 2014

    11.5K
    Quantifying Microorganisms at Low Concentrations Using Digital Holographic Microscopy DHM
    07:27

    Quantifying Microorganisms at Low Concentrations Using Digital Holographic Microscopy DHM

    Published on: November 1, 2017

    9.8K
  • Applied spatial filtering techniques to enhance image contrast.
  • Main Results:

    • Successfully demonstrated dynamic imaging of objects hidden within turbid media.
    • Achieved enhanced image contrast and improved focusing through the developed techniques.
    • Obtained clear dynamic images, validating the effectiveness of digital holography in this context.

    Conclusions:

    • Digital holography is a viable technique for dynamic imaging through turbid media.
    • The integration of autofocus and spatial filtering significantly improves imaging performance.
    • This method offers a promising non-ionizing approach for imaging in scattering environments.