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Development of a Lateral Flow Immunochromatographic Strip for Rapid and Quantitative Detection of Small Molecule Compounds
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A lateral flow paper microarray for rapid allergy point of care diagnostics
Thiruppathiraja Chinnasamy1, Loes I Segerink, Mats Nystrand
1Division of Proteomics and Nanobiotechnology, Science for Life Laboratory, KTH-Royal Institute of Technology, P-Box 1031, 171 21 Solna, Stockholm, Sweden. helene.andersson.svahn@scilifelab.se.
The Analyst
|April 3, 2014
Summary
A new lateral flow microarray device offers a convenient, low-cost method for detecting 15 specific IgE responses. This assay shows promise as an alternative to expensive multiplexed allergy tests for thresholds above 1 kU.
Area of Science:
- Allergy diagnostics
- Immunotechnology
- Biosensor development
Background:
- Existing multiplexed specific IgE tests are often costly or require complex instrumentation.
- There is a need for accessible and accurate methods to assess patient sensitization profiles.
- Current diagnostic tools may not meet the demand for high-throughput, cost-effective allergy screening.
Purpose of the Study:
- To develop and validate a novel, convenient lateral flow microarray (LFM) device for multiplexed specific IgE detection.
- To evaluate the sensitivity, specificity, and inter-assay variability of the new LFM assay.
- To compare the performance of the developed assay against a commercial standard (ImmunoCAP) using clinical samples.
Main Methods:
- A dual-labeled gold nanoparticle strategy was employed, conjugating HRP and anti-IgE to nanoparticles for enhanced enzymatic amplification.
- The LFM device was designed for the rapid detection of 15 specific IgE responses in clinical serum samples.
- Assay performance was assessed using 35 clinical samples, with results compared to ImmunoCAP, and read-out performed using a consumer flatbed scanner.
Main Results:
- The developed LFM assay demonstrated significant enzymatic amplification, improving sensitivity compared to commercial reagents.
- Mean inter-assay variability was found to be 12% coefficient of variation (CV).
- The assay showed good general agreement with ImmunoCAP, with varying performance across allergens (AUC = 0.54-0.88) for thresholds above 1 kU.
Conclusions:
- The novel LFM assay provides a rapid, sensitive, and low-cost method for multiplexed specific IgE detection.
- The assay's convenience, inexpensive materials, and use of a standard scanner make it a viable alternative for allergy diagnostics.
- This technology is particularly suitable for clinical settings where cost-effectiveness and ease of use are prioritized, especially for thresholds exceeding 1 kU.
