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Rapid Dissection and Dissociation of the Mouse Olfactory Epithelium for Single-Nucleus Suspensions
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Focussing on neuronal differentiation in the olfactory epithelium of the developing mouse

C Lehner1, A Wagner2, H Tempfer1

  • 1Paracelsus Private Medical University, Applied Cell Biology , Salzburg, Austria.

Journal of Stem Cells & Regenerative Medicine
|April 3, 2014
PubMed
Abstract

No abstract available in PubMed .

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The sense of smell is achieved through the activities of the olfactory system. It starts when an airborne odorant enters the nasal cavity and reaches olfactory epithelium (OE). The OE is protected by a thin layer of mucus, which also serves the purpose of dissolving more complex compounds into simpler chemical odorants. The size of the OE and the density of sensory neurons varies among species; in humans, the OE is only about 9-10 cm2.
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The process of olfaction, also known as the sense of smell, is a sophisticated chemical response system. The specialized sensory neurons that facilitate this process, known as olfactory receptor neurons, are situated in an upper segment of the nasal cavity, known as the olfactory epithelium. Olfactory sensory neurons are bipolar, with their dendrites extending from the epithelium's apex into the mucus that lines the nasal cavity. Airborne molecules, when inhaled, traverse the olfactory...
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