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Apoptosis induced by microwave radiation in pancreatic cancer JF305 cells

Wenhe Zhu1, Wei Zhang, Huiyan Wang

  • 1a Department of Biochemistry, Ji Lin Medical College, Ji Lin 132013, China.

Canadian Journal of Physiology and Pharmacology
|April 9, 2014
PubMed

Insights

Microwaves show promise in treating pancreatic cancer by inhibiting JF305 cell growth and inducing apoptosis. This effect is dose-dependent and mediated through a mitochondria-dependent pathway involving NF-κB signaling.

Area of Science:

  • Oncology
  • Biophysics

Background:

  • Pancreatic cancer remains a highly lethal malignancy with limited therapeutic options.
  • Novel treatment strategies are urgently required to improve patient survival rates.

Purpose of the Study:

  • To investigate the anti-cancer effects of microwave irradiation on pancreatic cancer JF305 cells.
  • To elucidate the underlying molecular mechanisms, including apoptosis induction and signaling pathways.

Main Methods:

  • JF305 cells were exposed to varying microwave doses (2.5-20.0 mW/cm²).
  • Cell proliferation was assessed using MTT assay.
  • Apoptosis was quantified via Hoechst staining, Nucleo-Counter, and Annexin V-FITC.
  • Protein expression (caspases, Bcl-2 family, NF-κB pathway) was analyzed by Western blot.

Main Results:

  • Microwave treatment inhibited JF305 cell proliferation in a dose-dependent manner.
  • Morphological changes indicative of apoptosis and increased apoptotic cell percentages were observed.
  • Microwaves modulated apoptosis-related proteins (increased caspase-9/3, Bax, CytoC; decreased Bcl-2) and NF-κB signaling (increased p65, decreased IκBα).

Conclusions:

  • Microwaves effectively inhibit pancreatic cancer cell growth and induce apoptosis.
  • The mechanism involves the activation of a mitochondria-mediated pathway regulated by NF-κB signaling.

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