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[Determination of catecholamines in blood plasma using high performance liquid chromatography on the "Millichrom"
Laboratornoe Delo
|January 1, 1989
Abstract:
The authors suggest a microcolumn modification for determining the blood plasma catecholamines by high performance liquid chromatography with electrochemical detection using a Soviet chromatograph Millikhrom. The analysis includes catecholamine extraction on aluminum oxide, elution with perchloric acid, and chromatography in a 60 x 2 mm column packed with 5 microns of Silasorb sph C8 sorbent. The method permits the detection of noradrenaline, adrenaline, and dopamine in concentrations 25-3200 pg/ml in 0.5 ml of blood plasma.