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Updated: May 1, 2026

Synthesis of Protein Bioconjugates via Cysteine-maleimide Chemistry
Published on: July 20, 2016
Design of maleimide-functionalised electrodes for covalent attachment of proteins through free surface cysteine
Emma J Wright1, Maciej Sosna, Sally Bloodworth
1Chemistry, Faculty of Natural and Environmental Sciences, University of Southampton, Southampton, SO17 1BJ (UK).
Abstract:
Mixed two-component monolayers on glassy carbon are prepared by electrochemical oxidation of N-(2-aminoethyl)acetamide and mono-N-Boc-hexamethylenediamine in mixed solution. Subsequent N-deprotection, amide coupling and solid-phase synthetic steps lead to electrode-surface functionalisation with maleimide, with controlled partial coverage of this cysteine-binding group at appropriate dilution for covalent immobilisation of a model redox-active protein, cytochrome c, with high coverage (≈7.5 pmol cm(-2) ).
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