Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Electrodes: Overview01:17

Electrodes: Overview

728
 Electrochemical measurements are conducted in an electrochemical cell composed of various components that control and measure the current and potential. One fundamental component is electrodes, conductive materials that enable electron transfer reactions at their surfaces.
There are two main types of electrodes in electrochemical cells. The first type, known as the working or indicator electrode, has a potential that is sensitive to the analyte's concentration and reacts to changes in...
728
Potentiometry: Membrane Electrodes01:15

Potentiometry: Membrane Electrodes

398
Membrane electrodes, also known as p-ion electrodes, use membranes that selectively interact with free analyte ions, generating a potential difference across the membrane. The resulting membrane potential, known as the asymmetry potential, is not zero even when analyte concentrations on both sides of the membrane are equal. The membrane's response is typically not selective to a single analyte but proportional to the concentration of all ions in the sample solution capable of interacting at...
398
Amperometry: Overview01:10

Amperometry: Overview

396
Amperometry is a technique commonly used to measure the concentration of specific analytes in a solution by monitoring the electric current generated during an electrochemical reaction. It involves applying a constant potential between a working electrode and a reference electrode to measure the resulting current, which is proportional to the concentration of the analyte. The Clark oxygen electrode operates based on this principle of amperometry. It consists of a cathode and an anode enclosed...
396
Potentiometry: Types of Electrodes01:19

Potentiometry: Types of Electrodes

474
Reference electrodes serve as a stable reference point for potentiometric measurements, while indicator and working electrodes react to variations in the composition of a solution.
The Standard Hydrogen Electrode (SHE) is a widely used reference electrode that maintains zero potential across all temperatures. However, its need for a continuous hydrogen gas supply renders it impractical for everyday use.
An alternative to SHE is the Saturated Calomel Electrode (SCE). This electrode features an...
474

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Trends in vibrational spectroscopy on optical waveguides.

Analytical and bioanalytical chemistry·2026
Same author

Community Mobilization to Promote Vaccine Confidence During a Global Public Health Emergency: Insights from Peel Region and Toronto (Ontario, Canada) a Qualitative Study.

Health services insights·2025
Same author

Probing defect formation in sulfur-annealed graphene for TMDC integration.

Nanoscale·2025
Same author

The role of trust in engaging community-based task forces and agencies among minoritized communities during a public health emergency.

Canadian journal of public health = Revue canadienne de sante publique·2025
Same author

Molecular precursors for the electrodeposition of 2D-layered metal chalcogenides.

Nature reviews. Chemistry·2025
Same author

Surface-enhanced Raman spectroscopy: a half-century historical perspective.

Chemical Society reviews·2024

Related Experiment Video

Updated: May 24, 2025

Fabrication of Amperometric Electrodes
08:29

Fabrication of Amperometric Electrodes

Published on: May 4, 2009

14.3K

Modelling enzyme electrodes - What do we learn and how is it useful?

Philip N Bartlett1, M Hashim Khan1

  • 1School of Chemistry and Chemical Engineering, University of Southampton, Southampton SO17 1BJ, UK.

Bioelectrochemistry (Amsterdam, Netherlands)
|February 28, 2025
PubMed
Summary

Approximate analytical models offer crucial insights into electrochemical systems, particularly for amperometric enzyme electrodes. These models help understand how factors like substrate concentration and enzyme properties influence current measurements.

Keywords:
Case diagramEnzyme electrodeModellingRedox-hydrogel

More Related Videos

Precise Electrochemical Sizing of Individual Electro-Inactive Particles
05:03

Precise Electrochemical Sizing of Individual Electro-Inactive Particles

Published on: August 4, 2023

1.1K
Modeling an Enzyme Active Site using Molecular Visualization Freeware
14:37

Modeling an Enzyme Active Site using Molecular Visualization Freeware

Published on: December 25, 2021

9.6K

Related Experiment Videos

Last Updated: May 24, 2025

Fabrication of Amperometric Electrodes
08:29

Fabrication of Amperometric Electrodes

Published on: May 4, 2009

14.3K
Precise Electrochemical Sizing of Individual Electro-Inactive Particles
05:03

Precise Electrochemical Sizing of Individual Electro-Inactive Particles

Published on: August 4, 2023

1.1K
Modeling an Enzyme Active Site using Molecular Visualization Freeware
14:37

Modeling an Enzyme Active Site using Molecular Visualization Freeware

Published on: December 25, 2021

9.6K

Area of Science:

  • Electrochemistry
  • Biomaterials
  • Computational Science

Background:

  • Significant advancements in computational power and modeling software have occurred since the 1960s.
  • Despite computational progress, approximate analytical treatments remain vital for understanding complex electrochemical systems.
  • Coupled diffusion/reaction problems are common in electrochemistry and require insightful modeling approaches.

Purpose of the Study:

  • To discuss the modeling of amperometric enzyme electrodes, specifically those utilizing redox hydrogel-based systems.
  • To explore the relationship between measured current and various experimental variables in these enzyme electrodes.
  • To highlight the utility of Case diagrams in analyzing coupled diffusion/reaction phenomena.

Main Methods:

  • Focus on approximate analytical treatments for modeling coupled diffusion/reaction problems.
  • Exemplar system: Redox hydrogel-based enzyme electrodes with immobilized enzymes.
  • Analysis of factors influencing current: substrate concentration, diffusion coefficient, reaction rates, film properties, and thickness.

Main Results:

  • Demonstrates the continued relevance of analytical methods alongside computational tools.
  • Illustrates how analytical models provide insights into system behavior.
  • Explains the interplay of multiple experimental variables affecting amperometric enzyme electrode performance.

Conclusions:

  • Approximate analytical treatments are indispensable for gaining fundamental understanding of electrochemical systems.
  • Case diagrams are valuable tools for dissecting complex diffusion/reaction dynamics in enzyme electrodes.
  • This approach aids in optimizing the design and application of amperometric enzyme electrodes.