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Updated: May 1, 2026

Protocol for Producing Three-Dimensional Infrared Video of Freezing in Plants
Published on: September 12, 2018
Ultrasound assisted immersion freezing of broccoli (Brassica oleracea L. var. botrytis L.)
Ying Xin1, Min Zhang2, Benu Adhikari3
1State Key Laboratory of Food Science and Technology, Jiangnan University, 214122 Wuxi, Jiangsu, China.
Objective:
The aim of this study was to research the ultrasound-assisted freezing (UAF) of broccoli.
Methods:
CaCl2 solution was used as freezing medium. The comparative advantage of using UAF over normal freezing on the freezing time, cell-wall bound calcium to total calcium ratio, textural properties, color, drip loss and L-ascorbic acid contents was evaluated.
Results:
The application of UAF at selected acoustic intensity with a range of 0.250-0.412 W/cm(2) decreased the freezing time and the loss of cell-wall bound calcium content. Compared to normal freezing, the values of textural properties, color, L-ascorbic acid content were better preserved and the drip loss was significantly minimized by the application of UAF. However, when outside that range of acoustic intensity, the quality of the ultrasound-assisted frozen broccoli was inferior compared to that of the normally frozen samples.
Conclusions:
Selected the appropriate acoustic intensity was very important for the application of UAF.
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