[A simplified method of counting microorganisms in studying human autoflora]

Area of Science:

• Microbiology
• Pediatric Health

Context:

• Assessing the microbiome of children is crucial for understanding health.
• Traditional methods for microbial enumeration can be resource-intensive.

Purpose:

• To derive formulae for calculating microorganism counts in pediatric bacterial biocenoses.
• To present a simplified drip method for enumerating microbial colonies in solid media.

Summary:

• New formulae have been derived for quantifying microorganisms in children's natural bacterial communities.
• A simplified drip method is proposed, reducing nutrient media use by over 3-fold and saving time.

Impact:

• This method offers a more efficient and cost-effective approach to microbial analysis in pediatric samples.
• Facilitates more accessible and widespread microbiome research in child health.