Alternative method for determination of contaminated heparin using chiral recognition
J Szekely1, M Collins1, C A Currie1
1College of Mount St. Joseph, Department of Chemistry, 5701 Delhi Road, Cincinnati, OH 45233, United States.
Summary
This study shows how to detect heparin contamination by analyzing chiral separations. Increased contamination negatively impacts pheniramine enantiomer separation but can improve chloroquine enantiomer resolution.
Area of Science:
- Analytical Chemistry
- Biochemistry
Background:
- Heparin analysis for contamination is crucial.
- Heparin's chiral properties offer a potential detection method.
Purpose of the Study:
- To investigate heparin's chiral selector capability for contamination analysis.
- To evaluate the impact of chondroitin sulfate contamination on enantiomer separations.
Main Methods:
- Chiral separations of pheniramine and chloroquine enantiomers using contaminated heparin.
- Calculation of electrophoretic mobility and resolution at varying contamination levels.
Main Results:
- Increased contamination decreased pheniramine enantiomer mobility and resolution linearly.
- Chloroquine enantiomer resolution improved at low contamination levels due to secondary interactions.
Conclusions:
- Chiral recognition is a viable method for detecting heparin contamination.
- Low-level chondroitin sulfate contamination can enhance chiral resolution for certain compounds.
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