Bem3: Filling the GAP between cell polarity and secretion
Arpita Sen1, Debarati Mukherjee2, R Claudio Aguilar1
1Department of Biological Sciences; Purdue University; West Lafayette, IN USA.
Communicative & Integrative Biology
|April 23, 2014
Summary
Vesicle trafficking regulates cell polarity by controlling the localization of Bem3, a Cdc42 regulator. This Bem3 compartment acts as a recycling station, interacting with the secretory pathway and recruiting Sec4.
Area of Science:
- Cell Biology
- Molecular Biology
- Biochemistry
Background:
- Cdc42, a Rho GTPase, is crucial for cell polarity.
- Vesicle trafficking is essential for cell polarity establishment and maintenance.
- Bem3, a GTPase activating protein for Cdc42, is implicated in polarity regulation.
Purpose of the Study:
- To investigate the role of vesicle trafficking in Bem3 localization and function.
- To elucidate the relationship between Bem3, Cdc42 signaling, and the secretory pathway.
- To explore the potential Spitzenkörper-like nature of the Bem3-containing compartment.
Main Methods:
- Observation of Bem3 localization and trafficking in budding yeast.
- Analysis of Bem3's interaction with actin tracks and polarized sites.
- Investigation of Bem3's recruitment of Sec4.
Main Results:
- Bem3 localizes to both the plasma membrane and an internal compartment.
- This compartment is involved in apical growth and acts as a recycling station.
- Bem3 actively recruits the secretory Rab GTPase Sec4, independent of its GAP activity.
Conclusions:
- Vesicle trafficking plays a key role in regulating cell polarity via Bem3 localization.
- A novel Spitzenkörper-like compartment involved in endocytic and secretory pathways has been identified.
- Bem3 and Sec4 exhibit complementary regulation, highlighting a link between polarity signaling and secretion.
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