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Utilizing a Comprehensive Immunoprecipitation Enrichment System to Identify an Endogenous Post-translational Modification Profile for Target Proteins
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Utilizing a Comprehensive Immunoprecipitation Enrichment System to Identify an Endogenous Post-translational Modification Profile for Target Proteins

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Human proteins with target sites of multiple post-translational modification types are more prone to be involved in

Qianli Huang1, Jinhui Chang, Man Kit Cheung

  • 1School of Life Sciences, The Chinese University of Hong Kong , Shatin, Hong Kong SAR 852000, China.

Journal of Proteome Research
|April 24, 2014
PubMed

Insights

Many proteins with multiple post-translational modifications (PTMs) are linked to diseases. These Mtp-proteins play crucial roles in cellular processes and disease development, often acting as network hubs.

Area of Science:

  • Molecular Biology
  • Systems Biology
  • Biochemistry

Background:

  • Proteins undergo post-translational modifications (PTMs) that regulate their function.
  • Proteins with multiple PTM types (Mtp-proteins) are increasingly associated with diseases like cancer.
  • Mechanisms linking Mtp-proteins to disease pathogenesis are not well understood.

Purpose of the Study:

  • To investigate the association between human Mtp-proteins and disease.
  • To systematically characterize the features of Mtp-proteins.
  • To explore the role of Mtp-proteins in disease development.

Main Methods:

  • Analysis of human Mtp-protein datasets.
  • Characterization of protein features, including PTM sites, protein-protein interactions (PPIs), and subcellular localization.
  • Examination of intrinsically disordered regions and PTM effects on PPI stability.

Main Results:

  • Mtp-proteins are significantly more involved in disease than proteins without PTMs.
  • Mtp-proteins are enriched in protein complexes, act as network hubs, and have specific functional roles (e.g., chromatin dynamics).
  • Combinations of PTMs dramatically alter protein-protein interaction stability, more so than single PTM events.

Conclusions:

  • Mtp-proteins are key players in disease development.
  • Understanding Mtp-protein characteristics offers insights into disease mechanisms.
  • PTM combinations significantly impact protein interactions and cellular functions relevant to disease.

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