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Further biodosimetry investigations using murine partial-body irradiation model
W F Blakely1, D J Sandgren2, V Nagy2
1Armed Forces Radiobiology Research Institute, 8901 Wisconsin Avenue, Bethesda, MD 20889-5603, USA william.blakely@usuhs.edu.
Radiation Protection Dosimetry
|April 24, 2014
Summary
This study on partial-body irradiation in mice found that lymphocyte counts decreased with increasing exposure. Physical restraint altered some blood cell counts and biomarker levels, impacting biological dosimetry.
Area of Science:
- Radiation biology
- Hematology
- Proteomics
Background:
- Biological dosimetry is crucial for assessing radiation exposure.
- Understanding the effects of partial-body irradiation and physical restraint on biomarkers is needed.
Purpose of the Study:
- To evaluate candidate biomarkers for biological dosimetry in a mouse model of partial-body irradiation.
- To assess the impact of physical restraint on these biomarkers post-irradiation.
Main Methods:
- CD2F1 male mice were subjected to partial-body irradiation (6-Gy, 250-kVp X ray) to different body fractions (3/3, 2/3, 1/3, 0/3).
- Blood samples were collected at 1 and 2 days post-exposure for hematological analysis and plasma proteomic biomarker assessment.
- The effects of physical restraint at 1 day post-exposure were also investigated.
Main Results:
- Increasing irradiated body fraction led to decreased lymphocyte counts and increased neutrophil-to-lymphocyte ratios.
- Plasma biomarker Flt3L showed proportional increases, while G-CSF and SAA levels increased dramatically and non-proportionally.
- Physical restraint modulated lymphocyte counts, SAA, neutrophil-to-lymphocyte ratio, and Flt3L levels, but not neutrophil or platelet counts or G-CSF.
Conclusions:
- Hematological parameters and specific plasma biomarkers (Flt3L, G-CSF, SAA) reflect the extent of partial-body irradiation.
- Physical restraint can influence these biological dosimetry markers, necessitating careful consideration in post-exposure assessments.
