Characteristic of fungal lipases and their application in synthesis of structered lipids

The experiments showed the possibility of the MLM type structured triacylglycerol synthesis. Such sTAGs should have polyene acids positioned in sn-2 (in this case evening primrose oil was the source of polyene acids) and caprylic acid in the sn-1 and sn-3 positions. The sTAG synthesis was carried out in two-stages: 1. alcoholysis and synthesis of 2-MAG; 2. 2-MAG esterification with caprylic acid. For further experiments 4 lipase preparations were selected: MCL, RDL, Lipozyme RM and TL. The lipase preparations synthesized 2-MAG with the yields of 85.7%,. 87.5%,. 54.3%,. 65.2%, respectively. The 2-MAGs separated by crystallization contained over 70% of C18:1 acid and over 7% of C18:3, n-6 acid. The two-staged sTAG synthesis was shown to be more efficient by analyzing the products composition. The two- stage process gave over 70% of MLM type triacylglycerols and about 3% of side-products, whereas the acidolysis or interesterification produced only about 30% MLM or MLL type triacylglycerols.