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Renal Tubule and Collecting Duct01:24

Renal Tubule and Collecting Duct

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The renal tubule is divided into three parts: the proximal convoluted tubule (PCT), the Loop of Henle (LOH), and the distal convoluted tubule (DCT).
Proximal Convoluted Tubule (PCT):
The PCT is the initial segment of the renal tubule, extending from the Bowman's capsule that encloses the glomerulus. Its convoluted structure and microvilli-lined cells increase the surface area for reabsorption. The PCT reabsorbs glucose, amino acids, sodium, and water from the filtrate, ensuring essential...
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Related Experiment Video

Updated: Apr 30, 2026

Nuclei Isolation from Adult Mouse Kidney for Single-Nucleus RNA-Sequencing
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Nuclei Isolation from Adult Mouse Kidney for Single-Nucleus RNA-Sequencing

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Validating single-cell genomics for the study of renal development.

Sanjay Jain1, Michiel J Noordam2, Masato Hoshi3

  • 11] Renal Division, Department of Internal Medicine, Washington University School of Medicine, St Louis, Missouri, USA [2] Department of Pathology and Immunology, Washington University School of Medicine, St Louis, Missouri, USA.

Kidney International
|April 25, 2014
PubMed
Summary
This summary is machine-generated.

Single-cell genomics shows promise for studying kidney development by accurately measuring gene expression in individual cells. This pilot study validates a new assay, revealing gene expression variations crucial for understanding kidney progenitor dynamics.

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Area of Science:

  • Developmental Biology
  • Genomics
  • Cell Biology

Background:

  • Single-cell genomics offers potential for investigating early kidney development.
  • Existing technologies require validation for accuracy and reproducibility in kidney progenitor studies.

Purpose of the Study:

  • To validate a high-throughput assay for measuring key kidney development gene expression in single embryonic mouse cells.
  • To assess the accuracy and reproducibility of single-cell expression data compared to traditional methods.

Main Methods:

  • A pilot study using a high-throughput assay on single cells and small cell pools from embryonic mouse ureteric buds.
  • Measurement of expression levels for 22 key kidney development genes.
  • Comparison of single-cell expression data with traditional microarray analysis (GUDMAP).

Main Results:

  • High concordance (correlation 0.82-0.85) between single-cell measurements and bulk tissue microarray data.
  • Discovery of significant variation in expression of Wnt11 and Etv5 in single cells, consistent with burst-like promoter kinetics.
  • Validation of the assay's ability to capture dynamic biological variations beyond population averages.

Conclusions:

  • The validated high-throughput assay is suitable for single-cell studies in kidney development.
  • Single-cell genomics can reveal dynamic gene expression patterns, such as burst-like kinetics, in kidney progenitors.
  • Future single-cell experiments informed by these findings will advance understanding of kidney development and disease.