[A chromatographic method of determining serotonin and histamine in a single sample of biological material]

Serotonin and histamine are important components of neurohumoral regulation. The biologic and fluorometric methods currently used for their measurements are labor-consuming and involve the use of unavailable reagents and expensive equipment. The authors suggest a sensitive simplified technique for serotonin and histamine measurements in a small volume of the biologic material (tissue and blood). The procedure is as follows: serotonin and histamine are isolated by thin-layer chromatography, the obtained samples are treated with alloxan in dimethyl formamide medium, and the stained solutions are examined in spectrophotometer. The suggested method is highly specific and sensitive, simple, makes use of Soviet equipment and of available reagents, this recommending it for clinical biochemical, as well as for experimental studies.