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A fluorescent light-up probe as an inhibitor of intracellular β-tryptase
Qi Wang1, Xiuyin Shi, Xiaoxia Zhu
1College of Public Health, Nantong University, 9 Seyuan Road, Nantong, 226019, China.
Chemical Communications (Cambridge, England)
|April 30, 2014
Abstract:
A pyrene-functionalized peptidic inhibitor binds to and inhibits β-tryptase in a non-competitive and reversible manner even in cells. Upon protein binding a fluorescence increase of the two pyrene fluorophores is observed which allows using as a fluorescent light-up probe for this enzyme.
