Related Experiment Video
Updated: Apr 30, 2026
07:32
Human Ex vivo Wound Model and Whole-Mount Staining Approach to Accurately Evaluate Skin Repair
Published on: February 17, 2021
10.1K
MicroRNAs in human skin ageing.
Mara Mancini1, Anna Maria Lena1, Gaelle Saintigny2
1University of "Tor Vergata", Department of Experimental Medicine and Surgery, Via Montpellier 1, Rome 00133, Italy.
Ageing Research Reviews
|May 3, 2014
Summary
Cellular senescence, marked by aging cells, reduces tissue repair. MicroRNAs are key regulators of this process in skin cells, offering insights into aging mechanisms.
Area of Science:
- Dermatology
- Cell Biology
- Gerontology
Background:
- Tissue regeneration declines with age, linked to accumulating senescent cells.
- Cellular senescence is a key factor in physiological aging.
- Understanding senescence mechanisms is crucial for aging research.
Purpose of the Study:
- To explore molecular mechanisms of cellular senescence in skin.
- To investigate the role of microRNAs in regulating skin cell senescence.
- To identify senescence-associated microRNAs and their targets in keratinocytes and dermal fibroblasts.
Main Methods:
- In vitro studies of keratinocytes and dermal fibroblasts.
- Induction of senescence via cellular stress (e.g., oxidative stress, telomere shortening).
- Analysis of microRNA regulation in replicative senescence.
Main Results:
- Senescence in skin cells (keratinocytes, fibroblasts) can be induced by various stresses.
- MicroRNAs play a critical role in balancing cell proliferation and senescence.
- Specific senescence-associated microRNAs and their targets were identified.
Conclusions:
- MicroRNAs are significant regulators of senescence in skin cells.
- Understanding these microRNA pathways can provide insights into skin aging.
- This research highlights potential targets for interventions in age-related tissue decline.
Related Concept Videos
01:12Renewal of Skin Epidermal Stem Cells
2.4K
The skin is divided into epidermis, dermis, and hypodermis, the skin's outermost, middle, and inner layers. The human epidermal layer regularly undergoes renewal, where old, dead cells are replaced by new cells. Epidermal stem cells or EpiSCs divide and differentiate to restore the lost cells. For the renewal process, some EpiSCs continuously self-renew. In contrast, few others differentiate into transit-amplifying cells, which later form prickle or spinous cells, followed by granular...
2.4K
01:27The Skin Microbiota
123
The human skin serves as a complex ecosystem inhabited by a diverse community of microorganisms, including bacteria, fungi, and viruses. This microbiome plays a critical role in maintaining skin health and defending against pathogenic invaders. The composition of microbial communities varies significantly across different regions of the body, influenced primarily by the local levels of moisture and sebum.Regional Variation in Skin MicrobiotaCutibacterium acnes predominantly colonizes sebaceous...
123
01:19The Effect of Aging on Tissues
3.6K
Several body functions deteriorate with age. The external signs of aging are easily identifiable. For example, the skin becomes dry, less elastic, and thins out, forming wrinkles. The skin of the face begins to appear looser due to a decrease in the levels of elastic and collagen fibers in the connective tissue. Additionally, melanin production in the hair follicle decreases with age, resulting in gray hair. Moreover, the senses of sight and hearing decline, so glasses and hearing aids may...
3.6K
01:23Tissue Renewal without Stem Cells
1.6K
After cellular or tissue damage, the resident stem cells present in the human body can locally repair and regenerate the damaged tissue or organ. However, even though some tissues do not have stem cells, they can repair and regenerate with the help of pre-existing cells. For example, beta cells of the pancreas and hepatocytes of the liver can divide to renew and regenerate the tissue. Here, both cell division and cell death are well regulated by homeostasis.
However, failure of such a system...
However, failure of such a system...
1.6K
01:37Mitochondria
13.4K
Mitochondria are eukaryotic cellular organelles that are known to produce energy through a process called oxidative phosphorylation. Besides their primary function, mitochondria are involved in various cellular processes, including cell growth, differentiation, signaling, metabolism, and senescence. Age-related changes cause a decline in mitochondrial quality and integrity due to increased mitochondrial mutations and oxidative damage. Thus, aging can severely impact mitochondrial functions,...
13.4K
01:22MicroRNAs
21.1K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
21.1K