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Related Concept Videos

In-situ Hybridization02:31

In-situ Hybridization

In situ hybridization (ISH) is a technique used to detect and localize specific DNA or RNA molecules in cells, tissue, or tissue sections using a labeled probe. The technique was first used in 1969 for the investigation of nucleic acids. It is currently an essential tool in scientific research and clinical settings, especially for diagnostic purposes.
Types of probes and labels
A probe is a complementary strand of DNA or RNA that binds to corresponding nucleotide sequences in a cell. Many...
FISH - Fluorescent In-situ Hybridization02:07

FISH - Fluorescent In-situ Hybridization

Fluorescence in situ hybridization, or FISH, was developed in the early 1980s and has quickly become one of the most widely used techniques in cytogenetics. Labeled probes are used to bind complementary DNA or RNA sequences on a chromosome or in a region within a cell. Earlier, the probes could only be obtained by cloning or reverse transcription of a DNA template. Currently, the probe oligonucleotides can be synthesized synthetically. Additionally, with the advancement of optical techniques,...
RNA-seq03:21

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while microarray-based...

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Related Experiment Video

Updated: May 19, 2026

RNA Fluorescence In Situ Hybridization for Long Non-Coding RNA Localization in Human Osteosarcoma Cells
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RNA Fluorescence In Situ Hybridization for Long Non-Coding RNA Localization in Human Osteosarcoma Cells

Published on: June 16, 2023

Identification of Long Noncoding RNA by In Situ Hybridization Approaches.

Mara Mancini1,2, Anna Maria Lena2, Eleonora Candi3,4

  • 1Department of Experimental Medicine, University of Rome "Tor Vergata", Rome, Italy.

Methods in Molecular Biology (Clifton, N.J.)
|May 18, 2026
PubMed
Summary
This summary is machine-generated.

This study details three RNA in situ hybridization methods to detect noncoding RNAs (ncRNAs) in human skin cells. These techniques, including RNAscope®, offer precise visualization of ncRNA expression and localization.

Keywords:
Fluorescent in situ hybridizationIn Situ hybridizationKeratinocytesLong noncoding RNANoncoding RNASkin

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Area of Science:

  • Molecular Biology
  • Genetics
  • Cell Biology

Background:

  • In situ hybridization (ISH) techniques are crucial for visualizing RNA expression within cells and tissues.
  • Detecting noncoding RNAs (ncRNAs) presents unique challenges due to their diverse functions and sometimes lower abundance.
  • Advances in RNA detection methodologies are needed to accurately profile ncRNAs in specific cellular contexts.

Purpose of the Study:

  • To provide detailed protocols for three distinct RNA in situ hybridization methods.
  • To optimize these methods for the specific detection of noncoding RNAs (ncRNAs).
  • To demonstrate the application of these techniques in normal human keratinocytes and skin tissue samples.

Main Methods:

  • In situ hybridization (ISH)
  • Fluorescent in situ hybridization (FISH)
  • RNAscope® technology for RNA detection

Main Results:

  • Successful adaptation of ISH, FISH, and RNAscope® for ncRNA detection in human keratinocytes.
  • Demonstration of specific RNA localization and expression patterns within skin tissue sections.
  • Comparative examples illustrating the capabilities of each method.

Conclusions:

  • The described in situ hybridization procedures effectively detect noncoding RNAs in human skin.
  • These optimized methods provide valuable tools for studying ncRNA roles in skin biology and disease.
  • RNAscope® offers a powerful approach for high-sensitivity ncRNA detection in fixed tissues.