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Related Concept Videos

Single Nucleotide Polymorphisms-SNPs01:05

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A single nucleotide polymorphism or SNP is a single nucleotide variation at a specific genomic position in a large population. It is the most prevalent type of sequence variation found in the human genome. Point mutations that occur in more than 1% of the population qualify as SNPs. These are present once every 1000 nucleotides on an average in the human genome. Replacement of a purine with another purine (A/G) or a pyrimidine with another pyrimidine (C/T) is known as a transition. In contrast,...
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Genome-wide Association Studies-GWAS01:11

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Genome-wide association studies or GWAS are used to identify whether common SNPs are associated with certain diseases. Suppose specific SNPs are more frequently observed in individuals with a particular disease than those without the disease. In that case, those SNPs are said to be associated with the disease. Chi-square analysis is performed to check the probability of the allele likely to be associated with the disease.
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Sequencing of the human genome has opened up several best-kept secrets of the genome. Scientists have identified thousands of genome variations that exist within a population. These variations can be a single nucleotide or a larger chromosomal variation.
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Candidate Gene Testing in Clinical Cohort Studies with Multiplexed Genotyping and Mass Spectrometry
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SNP genotyping: the KASP assay.

Chunlin He1, John Holme, Jeffrey Anthony

  • 1Generation Challenge Programme, CIMMYT, Km 45 Carretera México-Veracruz, El Batán, Texcoco, Estado de México, CP, 56130, Mexico, c.he@cgiar.org.

Methods in Molecular Biology (Clifton, N.J.)
|May 13, 2014
PubMed
Summary
This summary is machine-generated.

The KASP genotyping assay uses allele-specific PCR and fluorescence detection to identify genetic variations like SNPs and InDels. This flexible technology is widely used in human, animal, and plant genetics research.

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Area of Science:

  • Genetics
  • Molecular Biology
  • Biotechnology

Background:

  • Genetic variation analysis is crucial for understanding heritable traits and diseases.
  • Accurate detection of single nucleotide polymorphisms (SNPs) and insertions/deletions (InDels) is essential in genetic research.

Purpose of the Study:

  • To introduce and describe the KASP genotyping assay.
  • To highlight its capabilities in identifying and measuring genetic variation at the nucleotide level.

Main Methods:

  • Utilizes competitive allele-specific PCR.
  • Incorporates a homogeneous, fluorescence-based reporting system.
  • Detects genetic variations including SNPs and InDels.

Main Results:

  • The KASP assay provides accurate identification and measurement of genetic variation.
  • It is compatible with various equipment platforms and microtiter plate formats (96, 384, 1,536-well).
  • Demonstrates flexibility in analyzing numerous SNPs and samples.

Conclusions:

  • KASP technology offers a versatile and reliable method for genotyping.
  • Its broad applicability spans human, animal, and plant genetics.
  • The assay is suitable for both large-scale and small-scale laboratory applications.