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In vitro culture for doubled haploids: tools for molecular breeding
Sue Broughton1, Parminder K Sidhu, Philip A Davies
1Department of Agriculture and Food, Government of WA, 3 Baron-Hay Court, South Perth, 6151, WA, Australia, sue.broughton@agric.wa.gov.au.
Methods in Molecular Biology (Clifton, N.J.)
|May 13, 2014
Abstract:
Doubled haploid (DH) techniques using immature microspores or embryos rescued in vitro following interspecific hybridization are important tools for breeding wheat and barley and for generating populations for gene mapping purposes. Here, we describe methods being used for the successful production of spring wheat and barley DHs in Australia.
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