Related Experiment Video
Updated: Apr 30, 2026

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy
Published on: December 9, 2013
Simultaneous imaging of cellular morphology and multiple biomarkers using an acousto-optic tunable filter-based
Elliot S Wachman1, Stanley J Geyer2, Joel M Recht1
1Gooch and Housego, Lakewood, New Jersey 08701.
Abstract:
An acousto-optic tunable filter (AOTF)-based multispectral imaging microscope system allows the combination of cellular morphology and multiple biomarker stainings on a single microscope slide. We describe advances in AOTF technology that have greatly improved spectral purity, field uniformity, and image quality. A multispectral imaging bright field microscope using these advances demonstrates pathology results that have great potential for clinical use.
Related Concept Videos
Super-resolution Fluorescence Microscopy
Three-Dimensional Microscopy in Microbiology

