Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Introduction to the Human Microbiota01:22

Introduction to the Human Microbiota

199
Microorganisms colonize various regions of the human body, including the mouth, nasal passages, throat, stomach, intestines, urogenital tract, and skin. The total number of microbial cells is estimated to range from 10¹³ to 10¹⁴—comparable to, or exceeding, the number of human somatic cells. This host–microbiome relationship has led to the conceptualization of humans as supraorganisms, wherein microbial communities perform vital roles in development, immunity,...
199
Development of Human Microbiota01:30

Development of Human Microbiota

61
The human microbiota begins developing at birth and undergoes continual change as we age. Infancy marks a critical period of microbial sensitivity, offering a “window of opportunity” during which beneficial microbes help mature the immune system. By age three, children typically develop a more stable and diverse microbial community. Newborns acquire microbes from their immediate environment; vaginal delivery favors maternal vaginal microbes, while cesarean births favor microbes from...
61
Scale-Up Processes01:14

Scale-Up Processes

105
The scale-up of microbial fermentation processes is essential in industrial biotechnology, allowing the transition from laboratory-scale experiments to commercial-scale production while aiming to maintain product yield and quality. This process requires meticulous adjustment of equipment design, process parameters, and contamination control strategies to accommodate increasing culture volumes.At the laboratory scale, cultures are typically maintained in 1 to 10-liter glass or autoclavable...
105
Development of the Oral Microbiota01:28

Development of the Oral Microbiota

65
The establishment of the oral microbiome begins before birth, challenging the long-held belief that the fetal oral cavity is sterile. The presence of oral microbes such as Streptococcus and Fusobacterium in amniotic fluid suggests that microbial exposure may occur in utero, potentially through translocation from the maternal oral or gastrointestinal tract. This early colonization primes the neonatal immune system and sets the stage for subsequent microbial succession. Maternal health,...
65
Microbiota of the Stomach and Small Intestine01:27

Microbiota of the Stomach and Small Intestine

75
The human gastrointestinal (GI) tract is characterized by distinct physicochemical conditions that shape its microbial communities. Among these, the stomach presents a particularly challenging environment for microbial colonization due to its highly acidic pH, ranging from 1 to 3. This extreme acidity effectively limits microbial density. However, certain acid-tolerant microorganisms are capable of surviving in this niche. Notably, Helicobacter pylori can colonize the gastric mucosa,...
75
Environmental Applications of Microorganisms01:30

Environmental Applications of Microorganisms

1.5K
Microorganisms play a pivotal role in maintaining ecosystem balance by recycling essential elements such as carbon, nitrogen, and phosphorus, as well as supporting processes like bioremediation, wastewater treatment, and biofuel production.Microbes in Elemental CyclesIn the carbon cycle, microorganisms decompose organic matter, releasing carbon dioxide via aerobic respiration. This carbon dioxide is subsequently used by photosynthetic organisms to synthesize organic compounds, closing the...
1.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

DNA Cut-Ligation Cyclization Surpasses Jacobson-Stockmayer J-Factor Expectations by over Threefold.

Biomolecules·2026
Same author

Probing the limits of genetic recoding using multi-omics-guided evolution.

Nature communications·2026
Same author

Architectural fragility of gene regulatory networks underlies hematopoietic stem cell aging.

bioRxiv : the preprint server for biology·2026
Same author

Programmable Nucleic Acid Sensing in Human Cells Using Circularizable ssDNA.

Nature communications·2026
Same author

Designing genome editing experiments with EditABLE.

Genome biology·2026
Same author

A platform to design and optimise fluorogenic scFvs for detection of interleukin 33.

Chemical science·2026

Related Experiment Video

Updated: Apr 29, 2026

Co-culture of Living Microbiome with Microengineered Human Intestinal Villi in a Gut-on-a-Chip Microfluidic Device
10:51

Co-culture of Living Microbiome with Microengineered Human Intestinal Villi in a Gut-on-a-Chip Microfluidic Device

Published on: August 30, 2016

22.2K

Recent progress in engineering human-associated microbiomes.

Stephanie J Yaung1, George M Church, Harris H Wang

  • 1Program in Medical Engineering Medical Physics, Harvard-MIT Health Sciences and Technology, Cambridge, MA, USA.

Methods in Molecular Biology (Clifton, N.J.)
|May 20, 2014
PubMed
Summary
This summary is machine-generated.

Engineering microbial communities in the human body is becoming possible with new molecular biology tools. These advances promise better diagnostics and therapies for diseases linked to the human microbiota.

More Related Videos

Bioreactor Assembly for Continuous Culture of Complex Fecal Communities
09:37

Bioreactor Assembly for Continuous Culture of Complex Fecal Communities

Published on: April 25, 2025

1.4K
Updated Protocol for the Assembly and Use of the Minibioreactor Array (MBRA)
09:38

Updated Protocol for the Assembly and Use of the Minibioreactor Array (MBRA)

Published on: September 5, 2025

1.1K

Related Experiment Videos

Last Updated: Apr 29, 2026

Co-culture of Living Microbiome with Microengineered Human Intestinal Villi in a Gut-on-a-Chip Microfluidic Device
10:51

Co-culture of Living Microbiome with Microengineered Human Intestinal Villi in a Gut-on-a-Chip Microfluidic Device

Published on: August 30, 2016

22.2K
Bioreactor Assembly for Continuous Culture of Complex Fecal Communities
09:37

Bioreactor Assembly for Continuous Culture of Complex Fecal Communities

Published on: April 25, 2025

1.4K
Updated Protocol for the Assembly and Use of the Minibioreactor Array (MBRA)
09:38

Updated Protocol for the Assembly and Use of the Minibioreactor Array (MBRA)

Published on: September 5, 2025

1.1K

Area of Science:

  • Microbiology
  • Genetics
  • Systems Biology

Background:

  • Molecular biology and genetics advancements enable engineering of biological systems.
  • The human-associated microbiota is a key target for manipulation due to its health and disease relevance.

Purpose of the Study:

  • To discuss recent advances in engineering human-associated microbial communities.
  • To highlight the potential of these engineered communities for diagnostics and therapeutics.

Main Methods:

  • Review of recent technological developments in microbiota analysis.
  • Discussion of perturbation technologies for microbial communities.

Main Results:

  • Progress in engineering single- and multicellular biological systems.
  • Emerging technologies facilitate analysis and manipulation of the human microbiota.

Conclusions:

  • Engineered human microbial communities hold significant potential for future health applications.
  • Advances are paving the way for realizing the full potential of manipulating the human microbiome.