Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
CRISPR01:59

CRISPR

Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced Short...
Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
What is Genetic Engineering?00:49

What is Genetic Engineering?

Overview

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Synthetic circuits for cell ratio control.

Nature·2026
Same author

Manufacturing-aware generative models enable petascale synthesis of designed DNA.

Nature biotechnology·2026
Same author

Nutrient requirements of organ-specific metastasis in breast cancer.

Nature·2026
Same author

A Simple, Ultrastable, and Cost-Effective Oxygen-Scavenging System for Long-Term DNA-PAINT Imaging.

Small (Weinheim an der Bergstrasse, Germany)·2025
Same author

Engineered base editors with reduced bystander editing through directed evolution.

Nature biotechnology·2025
Same author

Plasmid2MC: efficient cell-free generation of high-purity minicircle DNA for genome editing in mammalian cells.

Communications biology·2025
Same journal

Integrated lipidomic and transcriptomic profiling of the host response in human malaria.

Genome biology·2026
Same journal

Centromeric satellite expansion drives genome evolution in the snowy owl.

Genome biology·2026
Same journal

Mapping the landscape of allele-specific expression in porcine genomes.

Genome biology·2026
Same journal

Genomic sequence evolution underlying human neocortical interareal diversification.

Genome biology·2026
Same journal

Regulatory mechanisms driven by functional 3'-UTR variants in alcohol use disorder and related traits.

Genome biology·2026
Same journal

A longitudinal single-nucleus transcriptomic atlas of bovine placentation reveals dynamic cellular hierarchies and regulatory programs.

Genome biology·2026
See all related articles

Related Experiment Video

Updated: May 12, 2026

Mouse Genome Engineering Using Designer Nucleases
12:04

Mouse Genome Engineering Using Designer Nucleases

Published on: April 2, 2014

Designing genome editing experiments with EditABLE.

Demetrios S Maxim1,2,3, Juliet Sostena3,4, Najani Shanee Johnson3,5

  • 1Division of Nephrology, Department of Medicine, Stanford University School of Medicine, Stanford, CA, USA.

Genome Biology
|May 11, 2026
PubMed
Summary
This summary is machine-generated.

EditABLE is a new online tool that centralizes CRISPR-Cas9 genome editing strategies. It predicts optimal editors and guide RNAs for base editing, prime editing, and integrase-mediated editing, aiding in genetic disorder research.

Keywords:
CRISPR–CasGene therapyGenome editingKidney disease

More Related Videos

Genome Editing in Mammalian Cell Lines using CRISPR-Cas
07:56

Genome Editing in Mammalian Cell Lines using CRISPR-Cas

Published on: April 11, 2019

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms
09:51

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms

Published on: May 25, 2018

Related Experiment Videos

Last Updated: May 12, 2026

Mouse Genome Engineering Using Designer Nucleases
12:04

Mouse Genome Engineering Using Designer Nucleases

Published on: April 2, 2014

Genome Editing in Mammalian Cell Lines using CRISPR-Cas
07:56

Genome Editing in Mammalian Cell Lines using CRISPR-Cas

Published on: April 11, 2019

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms
09:51

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms

Published on: May 25, 2018

Area of Science:

  • Genomics
  • Bioinformatics
  • Molecular Biology

Background:

  • CRISPR-Cas9 technology offers powerful genome editing capabilities.
  • Existing computational tools for CRISPR experiment design are fragmented.
  • A centralized resource is needed to optimize genome editing strategies.

Purpose of the Study:

  • To develop EditABLE, an integrated online platform for designing efficient CRISPR-Cas genome editing strategies.
  • To provide a centralized resource for selecting optimal CRISPR editors and guide RNAs.
  • To facilitate applications in base editing, prime editing, and integrase-mediated editing.

Main Methods:

  • Developed EditABLE, an accessible web-based application (EditABLE-app.stanford.edu).
  • Integrated functionalities for base editing, prime editing, and integrase-mediated editing.
  • Utilized user-provided sequence data for personalized editing strategy prediction.

Main Results:

  • EditABLE successfully identifies optimal CRISPR editors and guide RNAs for specific genome editing applications.
  • The platform demonstrates utility in targeting complex genetic disorders, exemplified by autosomal dominant polycystic kidney disease (ADPKD).
  • Specific editing tools were identified across the ADPKD mutation landscape, showcasing EditABLE's practical application.

Conclusions:

  • EditABLE serves as a valuable centralized resource for designing efficient CRISPR-Cas genome editing experiments.
  • The platform streamlines the selection of appropriate editing tools for diverse applications, including base editing, prime editing, and integrase-mediated editing.
  • EditABLE aids in advancing research for genetic disorders by providing tailored editing strategies.