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Related Concept Videos

DNA Isolation01:24

DNA Isolation

DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...

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A Simple, Ultrastable, and Cost-Effective Oxygen-Scavenging System for Long-Term DNA-PAINT Imaging.

Rebecca T Perelman1,2, George M Church1,3, Johannes Stein1,3,4

  • 1Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, Massachusetts, USA.

Small (Weinheim an Der Bergstrasse, Germany)
|December 19, 2025
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Summary

Researchers developed a new enzyme-free buffer using sodium sulfite and Trolox to prevent damage during DNA-PAINT super-resolution microscopy. This stable, cost-effective solution significantly improves long-term imaging performance by protecting DNA structures.

Keywords:
DNA‐PAINToxygen‐scavengersingle‐moleculesuper‐resolution microscopy

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Area of Science:

  • Biophysics
  • Microscopy
  • Molecular Biology

Background:

  • DNA-PAINT is a super-resolution technique enabling nanoscale imaging.
  • It relies on transient DNA hybridization, offering photobleaching resistance.
  • Extended imaging is limited by reactive oxygen species (ROS) damaging DNA structures.

Purpose of the Study:

  • To develop a robust, enzyme-free oxygen scavenging system (OSS) for DNA-PAINT.
  • To overcome limitations of current enzymatic OSS, such as degradation and reduced performance.
  • To enhance long-term DNA-PAINT imaging stability and cost-effectiveness.

Main Methods:

  • Formulation of an enzyme-free buffer combining sodium sulfite (Na2SO3) and Trolox (SST).
  • Testing the preservation of DNA docking strand integrity over extended periods (24+ hours).
  • Evaluating the impact of the SST buffer on DNA-PAINT imaging performance and stability.

Main Results:

  • The SST buffer effectively preserves docking strand integrity for over 24 hours.
  • It significantly enhances long-term DNA-PAINT imaging performance.
  • SST demonstrates tenfold improvement in buffer stability, ease of preparation, and over 90% cost reduction.

Conclusions:

  • The enzyme-free SST buffer provides a robust, cost-effective, and high-performance OSS for DNA-PAINT.
  • This formulation overcomes the limitations of enzymatic OSS, enabling extended super-resolution imaging.
  • SST offers a practical solution for improving the reliability and accessibility of DNA-PAINT microscopy.