Highly sensitive and selective determination of bisphenol-A using peptide-modified gold electrode

Area of Science:

• Analytical Chemistry
• Biotechnology
• Materials Science

Background:

• Accurate determination of bisphenol A (BPA) is crucial due to its widespread presence and potential health concerns.
• Existing methods for BPA detection can be time-consuming or lack the required specificity for complex matrices.

Purpose of the Study:

• To develop a novel electrochemical biosensor for the rapid and selective detection of bisphenol A (BPA).
• To utilize a specifically isolated peptide as the recognition element for BPA detection.

Main Methods:

• Isolation of a BPA-specific peptide (Cys-Lys-Ser-Leu-Glu-Asn-Ser-Tyr-Cys, CKSLENSYC) using phage display.
• Construction of an electrochemical biosensor on a gold electrode using the affinity peptide.
• Characterization of the peptide monolayer using ATR-IR, CV, and EIS.
• Optimization of sensing conditions and BPA determination using differential pulse voltammetry (DPV).

Main Results:

• Successful immobilization of a self-assembled peptide monolayer on the gold electrode.
• The developed biosensor achieved a detection time of under 5 minutes.
• Demonstrated a broad dynamic detection range of 1-5000 nM for BPA.
• Exhibited high sensitivity and selectivity, with minimal interference from phenolic compounds and inorganic ions.

Conclusions:

• The novel peptide-based electrochemical biosensor offers a fast, sensitive, and selective method for BPA determination.
• This technology has potential applications in environmental monitoring and food safety testing.
• The use of affinity peptides provides a promising strategy for developing advanced biosensing platforms.