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Visualization of Organelles In Situ by Cryo-STEM Tomography
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The spatial coherence function in scanning transmission electron microscopy and spectroscopy.

D T Nguyen1, S D Findlay2, J Etheridge3

  • 1Dept. of Materials Engineering, Monash University, VIC 3800, Australia.

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|June 1, 2014
PubMed
Summary
This summary is machine-generated.

The effective source distribution significantly impacts scanning transmission electron microscopy (STEM) image contrast and signal origin. Understanding this spatial coherence is crucial for accurate atomic-resolution analysis.

Keywords:
ADFChannellingPartial spatial coherenceSTEM

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Area of Science:

  • Materials Science
  • Physics
  • Chemistry

Background:

  • Quantitative analysis in scanning transmission electron microscopy (STEM) relies on understanding signal origins.
  • The spatial coherence function, or effective source distribution, is critical for interpreting STEM data.

Purpose of the Study:

  • To investigate how the spatial coherence function's form affects quantitative analysis in STEM.
  • To determine the influence of effective source distribution on imaging and spectroscopy signal origins.

Main Methods:

  • Utilized three distinct source distribution models.
  • Applied these models to a Gallium Arsenide (GaAs) crystal case study.
  • Analyzed the impact on scanning transmission electron microscopy (STEM) image contrast and scattered electron wavefield.

Main Results:

  • The effective source distribution shape strongly influences STEM image contrast.
  • It also affects the scattered electron wavefield distribution and detected electron intensities' spatial origin.
  • Demonstrated significant impact on atomic-resolution measurements.

Conclusions:

  • The effective source distribution is a key factor in quantitative STEM analysis.
  • Accurate interpretation of structure, composition, and bonding requires considering the source distribution.
  • This impacts annular dark field, X-ray, and electron energy loss STEM imaging.