Account: characterization and identification of microcystins by mass spectrometry

In this brief overview, the authors describe mass spectral techniques for the detection and identification of microcystin toxins. Microcystins are secondary metabolites produced by cyanobacteria. Determination of these toxic compounds and discovery of new variants is very important as they pose a great danger to the human food chain. Cyanobacterial blooms frequently occur in many areas worldwide and have the potential to contaminate the water via cyanotoxin release, especially microcystins. Among the various analytical techniques used for analysis, mass spectrometry has become the most important method as it allows simultaneous quantification and structural characterization of multiple microcystin variants. This brief overview article focuses on mass spectrometry techniques for identification of microcystins, including ionization methods, mass spectral fragmentation routes, profiling techniques, tandem and high-resolution mass spectrometry as well as typing of cyanobacterial strains.