Biosensing platform based on graphene oxide via self-assembly induced by synergic interactions

Area of Science:

• Biomedical Engineering
• Materials Science
• Analytical Chemistry

Background:

• Development of sensitive and reliable glucose biosensors is crucial for diabetes management.
• Graphene oxide (GO) offers unique properties for biosensor development due to its large surface area and excellent conductivity.
• Efficient immobilization of enzymes onto the GO surface is key to biosensor performance.

Purpose of the Study:

• To construct a novel self-assembled glucose biosensor using graphene oxide (GO).
• To investigate the use of 1-pyrenebutyric acid-N-hydroxysuccinimide ester (PANHS) as a linking molecule for enzyme immobilization.
• To characterize the biosensor's performance, including its analytical range, response time, sensitivity, and stability.

Main Methods:

• Stepwise self-assembly of PANHS onto GO in DMF, followed by glucose oxidase (GOD) binding in aqueous solution.
• Characterization of molecular interactions and morphology using FTIR, FESEM, and AFM.
• Quantitative analysis of PANHS and GOD loading via surface plasmon resonance (SPR).

Main Results:

• Successful construction of a self-assembled glucose biosensor based on GO.
• Demonstrated wide linear range (4.0×10⁻⁶ to 4.4×10⁻³ M) and fast response time (10s).
• Achieved high sensitivity (40.5±0.4 mA M⁻¹ cm⁻²) and a low detection limit (2 μM, S/N=3).
• Exhibited excellent long-term stability and satisfactory reproducibility.

Conclusions:

• The novel self-assembled glucose biosensor demonstrates excellent analytical performance.
• The PANHS linking molecule facilitates efficient GOD immobilization on GO.
• This biosensor shows significant potential for accurate and reliable glucose detection.