Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Genetic variation in an miRNA-1827 binding site in MYCL1 alters susceptibility to small-cell lung cancer.

Cancer research·2011
Same author

Effects of electrode surface modification with chlorotoxin on patterning single glioma cells.

Physical chemistry chemical physics : PCCP·2011
Same author

Intracranial clear cell meningioma: a clinicopathologic study of 15 cases.

Acta neurochirurgica·2011
Same author

Striatal-enriched protein tyrosine phosphatase expression and activity in Huntington's disease: a STEP in the resistance to excitotoxicity.

The Journal of neuroscience : the official journal of the Society for Neuroscience·2011
Same author

1-(4,5-Dinitro-10-aza-tricyclo-[6.3.1.0]dodeca-2,4,6-trien-10-yl)-2,2,2-trifluoro-ethanone.

Acta crystallographica. Section E, Structure reports online·2011
Same author

1,2,3,4-Tetra-hydro-1,4-methano-naphthalene-2,3-diol.

Acta crystallographica. Section E, Structure reports online·2011

Related Experiment Video

Updated: Apr 28, 2026

Transfection of Mouse Retinal Ganglion Cells by in vivo Electroporation
05:26

Transfection of Mouse Retinal Ganglion Cells by in vivo Electroporation

Published on: April 17, 2011

15.0K

Genetically targeted binary labeling of retinal neurons.

Yongling Zhu1, Jian Xu2, William W Hauswirth3

  • 1Departments of Ophthalmology and Physiology, Northwestern University Feinberg School of Medicine, Chicago, Illinois 60611, and yongling-zhu@northwestern.edu s-devries@northwestern.edu.

The Journal of Neuroscience : the Official Journal of the Society for Neuroscience
|June 6, 2014
PubMed
Summary

Researchers developed a new sparse labeling technique to study diverse neural circuits. This method precisely identifies and characterizes specific interneuron types, revealing new cell populations in the retina and enabling broader circuit analysis.

Keywords:
Cre recombinaseamacrine cellganglion cellrabies virusretina

More Related Videos

Time-Lapse Imaging of Neuronal Arborization using Sparse Adeno-Associated Virus Labeling of Genetically Targeted Retinal Cell Populations
13:13

Time-Lapse Imaging of Neuronal Arborization using Sparse Adeno-Associated Virus Labeling of Genetically Targeted Retinal Cell Populations

Published on: March 19, 2021

2.5K
Targeted Labeling of Neurons in a Specific Functional Micro-domain of the Neocortex by Combining Intrinsic Signal and Two-photon Imaging
11:24

Targeted Labeling of Neurons in a Specific Functional Micro-domain of the Neocortex by Combining Intrinsic Signal and Two-photon Imaging

Published on: December 12, 2012

12.9K

Related Experiment Videos

Last Updated: Apr 28, 2026

Transfection of Mouse Retinal Ganglion Cells by in vivo Electroporation
05:26

Transfection of Mouse Retinal Ganglion Cells by in vivo Electroporation

Published on: April 17, 2011

15.0K
Time-Lapse Imaging of Neuronal Arborization using Sparse Adeno-Associated Virus Labeling of Genetically Targeted Retinal Cell Populations
13:13

Time-Lapse Imaging of Neuronal Arborization using Sparse Adeno-Associated Virus Labeling of Genetically Targeted Retinal Cell Populations

Published on: March 19, 2021

2.5K
Targeted Labeling of Neurons in a Specific Functional Micro-domain of the Neocortex by Combining Intrinsic Signal and Two-photon Imaging
11:24

Targeted Labeling of Neurons in a Specific Functional Micro-domain of the Neocortex by Combining Intrinsic Signal and Two-photon Imaging

Published on: December 12, 2012

12.9K

Area of Science:

  • Neuroscience
  • Cell Biology
  • Genetics

Background:

  • Understanding neural circuits is hindered by the diverse nature of interneurons.
  • Current Cre driver mouse lines often lack specificity, limiting targeted manipulation of interneuron subtypes.
  • A method for precise, sparse labeling of specific interneuron populations is crucial for detailed circuit analysis.

Purpose of the Study:

  • To develop and validate a novel sparse labeling strategy for precise identification and characterization of interneuron subtypes.
  • To apply this technique to GABAergic Cre mouse lines for analyzing retinal amacrine and ganglion cells.
  • To demonstrate the broad applicability of the method for neuronal circuit mapping and manipulation in various brain regions.

Main Methods:

  • Combination of Cre driver mice, recombinant adeno-associated virus (AAV), and rabies virus for sparse, binary neuronal labeling.
  • Application of the technique to GABAergic Cre mouse lines (Mus musculus) to study retinal cell types.
  • Utilizing antibody staining for GABA and characterizing Cre-expressing cells in the retina, cortex, lateral geniculate nucleus, and superior colliculus.

Main Results:

  • Successful sparse and binary labeling of select interneurons, often resulting in single-cell resolution.
  • Identification of two novel amacrine cell types in the retina: an asymmetric medium-field type and a wide-field type.
  • Characterization of previously identified wide-field amacrine cells lacking specific genetic markers and GABA-expressing amacrine cells, as well as non-GABAergic retinal ganglion cells (RGCs).

Conclusions:

  • The developed sparse labeling approach effectively overcomes limitations of existing Cre driver lines for studying neuronal diversity.
  • This technique provides unprecedented genetic access to specific, hard-to-access neuronal populations for comprehensive anatomical, physiological, and functional studies.
  • The method is versatile and applicable to various brain regions and for expressing optogenetic tools and protein sensors, advancing neural circuit mapping and manipulation.