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Updated: Apr 28, 2026

Fluorescence Microscopy Methods for Determining the Viability of Bacteria in Association with Mammalian Cells
Published on: September 5, 2013
The use of lysozyme modified with fluorescein for the detection of Gram-positive bacteria
Michał Arabski1, Iwona Konieczna1, Ewa Tusińska1
1Department of Microbiology, Jan Kochanowski University in Kielce, Świętokrzyska 15, 25-406 Kielce, Poland.
Abstract:
Lysozyme (1,4-β-N-acetylmuramidase) is commonly applied in the food, medical, and pharmaceutical industries. In this study, we tested a novel application of fluorescein-modified lysozyme (using carboxyfluorescein with a triazine-based coupling reagent) as a new tool for the detection of Gram-positive soil bacteria. The results, obtained by cultivation methods, fluorescence analysis, and laser interferometry, showed that, after optimization, fluorescein-modified lysozyme could be used to evaluate the prevalence of Gram-positive bacteria essential in bioremediation of soils with low pH, such as those degraded by sulfur.
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