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Decreased D2-40 immunoreactivity in stored paraffin sections and methods for preserving it
T Sasaki1, Y Kawabata, N Suzuki
1Department of Anatomy and Neuroscience, Hamamatsu University School of Medicine , 1 Handayama, Higashi-ku, Hamamatsu, Shizuoka , Japan.
Summary
Long-term storage of tissue sections can weaken D2-40 antibody staining. Preserving sections at low temperatures (4°C or -30°C) improves D2-40 immunostaining quality for lymphatic vessel research.
Area of Science:
- Immunohistochemistry
- Lymphatic Biology
- Pathology
Background:
- D2-40, an antibody targeting podoplanin, is a key marker for lymphatic endothelium.
- It is crucial for lymphatic vessel research and diagnosing lymphatic pathologies.
Purpose of the Study:
- To investigate how tissue section storage duration affects D2-40 antibody immunostaining.
- To evaluate the impact of different preservation methods on D2-40 staining over time.
Main Methods:
- Examined D2-40 immunostaining in scalp skin and lymph node sections stored for varying durations.
- Compared staining results after room temperature storage versus low-temperature storage (4°C and -30°C).
- Assessed staining with NZ-1 and Lyve-1 antibodies on preserved sections.
Main Results:
- Long-term room temperature storage attenuated D2-40 staining.
- Low-temperature preservation (4°C or -30°C) significantly improved D2-40 staining in stored sections.
- NZ-1 and Lyve-1 antibodies showed clear staining even after 16 weeks of storage.
Conclusions:
- Avoid long-term storage of D2-40 immunostained tissue sections at room temperature.
- Preserve D2-40 stained sections at low temperatures to maintain staining integrity for research and diagnostics.
