Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

MALDI-TOF Mass Spectrometry01:19

MALDI-TOF Mass Spectrometry

5.8K
Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.Matrix-assisted laser desorption ionization (MALDI) is a commonly...
5.8K
Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

6.2K
Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
6.2K
Matrix-Assisted Laser Desorption Ionization (MALDI)01:08

Matrix-Assisted Laser Desorption Ionization (MALDI)

1.4K
Matrix-assisted laser desorption ionization (MALDI) is a powerful analytical technique used in mass spectrometry. It enables the identification and characterization of various biomolecules, including proteins, peptides, nucleic acids, and carbohydrates. MALDI is an ionization technique, widely employed in biological and medical research, as well as in fields like pharmacology and biochemistry.The analyte of interest, a biomolecule or a mixture of biomolecules, is mixed with a suitable matrix...
1.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

From Veterinary Medicine to Illicit Drug Supply: Utilising Social Media to Explore the Rising Emergence of Veterinary Medicines in Human Health.

Brain sciences·2025
Same author

Exploring Human Misuse and Abuse of Veterinary Drugs: A Descriptive Pharmacovigilance Analysis Utilising the Food and Drug Administration's Adverse Events Reporting System (FAERS).

Toxics·2024
Same author

Exploring the Confluence of Animal Medicine and its Implications for Human Health: A Systematic Literature Review.

Current neuropharmacology·2024
Same author

The effect of 8-day oral taurine supplementation on thermoregulation during low-intensity exercise at fixed heat production in hot conditions of incremental humidity.

European journal of applied physiology·2024
Same author

Embedding retrieval practice in undergraduate biochemistry teaching using PeerWise.

Biochemistry and molecular biology education : a bimonthly publication of the International Union of Biochemistry and Molecular Biology·2023
Same author

Antimicrobial Volatiles of the Insect Pathogen <i>Metarhizium brunneum</i>.

Journal of fungi (Basel, Switzerland)·2022

Related Experiment Video

Updated: Apr 27, 2026

Using the Open-Source MALDI TOF-MS IDBac Pipeline for Analysis of Microbial Protein and Specialized Metabolite Data
09:29

Using the Open-Source MALDI TOF-MS IDBac Pipeline for Analysis of Microbial Protein and Specialized Metabolite Data

Published on: May 15, 2019

20.7K

MALDI profiling and applications in medicine.

Ed Dudley1

  • 1Institute of Mass Spectrometry, College of Medicine, Swansea University, Singleton Park, Swansea, UK, e.dudley@swansea.ac.uk.

Advances in Experimental Medicine and Biology
|June 22, 2014
PubMed
Summary
This summary is machine-generated.

Matrix-assisted laser desorption ionization (MALDI) mass spectrometry rapidly profiles biomolecules in tissues and fluids. Its applications extend from protein identification to advanced spatial analysis in biomedicine.

More Related Videos

Whole-cell MALDI-TOF Mass Spectrometry is an Accurate and Rapid Method to Analyze Different Modes of Macrophage Activation
11:25

Whole-cell MALDI-TOF Mass Spectrometry is an Accurate and Rapid Method to Analyze Different Modes of Macrophage Activation

Published on: December 26, 2013

8.4K
Sample Preparation for Metabolic Profiling using MALDI Mass Spectrometry Imaging
09:08

Sample Preparation for Metabolic Profiling using MALDI Mass Spectrometry Imaging

Published on: December 22, 2020

6.6K

Related Experiment Videos

Last Updated: Apr 27, 2026

Using the Open-Source MALDI TOF-MS IDBac Pipeline for Analysis of Microbial Protein and Specialized Metabolite Data
09:29

Using the Open-Source MALDI TOF-MS IDBac Pipeline for Analysis of Microbial Protein and Specialized Metabolite Data

Published on: May 15, 2019

20.7K
Whole-cell MALDI-TOF Mass Spectrometry is an Accurate and Rapid Method to Analyze Different Modes of Macrophage Activation
11:25

Whole-cell MALDI-TOF Mass Spectrometry is an Accurate and Rapid Method to Analyze Different Modes of Macrophage Activation

Published on: December 26, 2013

8.4K
Sample Preparation for Metabolic Profiling using MALDI Mass Spectrometry Imaging
09:08

Sample Preparation for Metabolic Profiling using MALDI Mass Spectrometry Imaging

Published on: December 22, 2020

6.6K

Area of Science:

  • Biochemistry
  • Analytical Chemistry
  • Biomedical Science

Background:

  • Matrix-assisted laser desorption ionization (MALDI) mass spectrometry is a powerful technique for analyzing biomolecules.
  • Originally used for protein and peptide analysis, its applications have expanded significantly.

Purpose of the Study:

  • To discuss the MALDI process and its established applications.
  • To highlight advancements in MALDI mass spectrometry for biomolecular profiling.
  • To explore the diverse biomedical applications of MALDI.

Main Methods:

  • MALDI mass spectrometry for rapid biomolecular profiling of biofluids and tissues.
  • Peptide mass fingerprinting for protein identification.
  • Imaging MALDI analysis for spatial distribution of biomolecules.

Main Results:

  • MALDI enables rapid profiling of diverse biomolecular species.
  • The technique is applicable to lipidomics, genotyping, microorganism identification, biomarker discovery, and metabolomics.
  • Imaging MALDI allows for the spatial mapping of biomolecules within samples.

Conclusions:

  • MALDI mass spectrometry is a versatile tool with broad applications in biomedicine.
  • Advances in MALDI continue to expand its utility in biomolecular analysis and spatial profiling.
  • The technique offers significant potential for biomarker discovery and diagnostics.