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Updated: Apr 27, 2026
09:06
MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as a Novel Detection and Quantification Method
Published on: October 7, 2025
552
MicroRNA methodology: advances in miRNA technologies.
Theresa Kaeuferle1, Sabine Bartel, Stefan Dehmel
1Dr. von Hauner'sche Kinderklinik, Klinikum der Universität München, 80337, Munich, Germany.
Methods in Molecular Biology (Clifton, N.J.)
|June 25, 2014
Summary
MicroRNA research is advancing rapidly, with new technologies enabling detailed studies. This work covers key methods for microRNA expression profiling, target identification, and functional validation, including in vivo applications.
Area of Science:
- Molecular Biology
- Genetics
- Biotechnology
Background:
- MicroRNAs (miRNAs) are small non-coding RNAs regulating gene expression.
- Emerging trends highlight significant advancements in miRNA research and technologies.
- Understanding miRNA functions requires robust experimental methodologies.
Purpose of the Study:
- To provide insights into key miRNA-specific methodologies.
- To detail critical steps in miRNA expression profiling and target gene identification.
- To explain functional confirmation and in vivo applications of miRNA modulation.
Main Methods:
- miRNA expression profiling techniques.
- Bioinformatic and experimental approaches for target gene identification.
- Functional assays for confirming miRNA effects.
- In vivo studies utilizing antagomirs for miRNA inhibition.
Main Results:
- Comprehensive overview of established and emerging miRNA research methodologies.
- Detailed explanation of critical steps in the miRNA analysis workflow.
- Demonstration of functional confirmation from cellular to in vivo levels.
Conclusions:
- The chapter offers a valuable resource for researchers in the miRNA field.
- It bridges the gap between basic research and therapeutic applications of miRNAs.
- Advances in miRNA technologies facilitate deeper understanding and manipulation of gene regulation.
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