Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Super-resolution Fluorescence Microscopy

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

IL-1β/EPAS1-Associated Ferroptotic Stress Impairs Skeletal Stem/Progenitor Cell Function in Inflammation-Associated Fracture Nonunion.

Current issues in molecular biology·2026

Same author

Timing of High-Risk Asthma Specialist Enrollment and Severe Asthma Exacerbations in Children.

The journal of allergy and clinical immunology. In practice·2026

Same author

Optimizing scalable approaches for early detection of cognitive impairment in primary care.

Alzheimer's & dementia (Amsterdam, Netherlands)·2026

Same author

Effect of an electronic health record-integrated machine learning asthma risk marker on pediatrician prognostic accuracy during preschool age: a pilot randomized clinical trial.

Scientific reports·2026

Same author

Early initiation of inhaled corticosteroid-long-acting β<sub>2</sub>-agonist therapy and reduction of severe asthma exacerbations in high-risk preschool children: A longitudinal real-world cohort study.

Pediatric allergy and immunology : official publication of the European Society of Pediatric Allergy and Immunology·2026

Same author

Authorship identification for Chinese literature based on a pyramid deep bidirectional gated recurrent unit network with voting strategy.

Scientific reports·2026

Same journal

Gaussian-modulated continuous-variable quantum key distribution over 60 km fiber using an integrated silicon photonic receiver.

Optics letters·2026

Same journal

E2E-OCT: end-to-end joint learning model using optical coherence tomography images for vocal cord leukoplakia diagnosis.

Optics letters·2026

Same journal

Holographic generation of panoramic 3D scenes by concave ellipsoidal mirror reflection.

Optics letters·2026

Same journal

Dual-pilot phase recovery with pair-wise maximum-ratio combining for coherent PONs.

Optics letters·2026

Same journal

Mapping the whispering gallery modes of a CaF<sub>2</sub> disk resonator with half-tapered fibers to estimate the fundamental mode volume.

Optics letters·2026

Same journal

Quantitative estimation of deep-subwavelength scale via dark-field scattering axial energy concentration decay profiles.

Optics letters·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Apr 27, 2026

Three-dimensional Optical-resolution Photoacoustic Microscopy

Three-dimensional Optical-resolution Photoacoustic Microscopy

Published on: May 3, 2011

Multiscale photoacoustic microscopy with continuously tunable resolution.

Bowen Jiang, Xiaoquan Yang, Yanyan Liu

|July 1, 2014

Summary

This summary is machine-generated.

Researchers developed a multiscale photoacoustic microscope with continuously tunable resolution. This innovation allows imaging from optical to acoustic scales, demonstrated in vivo on a mouse ear.

More Related Videos

Switchable Acoustic and Optical Resolution Photoacoustic Microscopy for In Vivo Small-animal Blood Vasculature Imaging

Switchable Acoustic and Optical Resolution Photoacoustic Microscopy for In Vivo Small-animal Blood Vasculature Imaging

Published on: June 26, 2017

Dual Raster-Scanning Photoacoustic Small-Animal Imager for Vascular Visualization

Dual Raster-Scanning Photoacoustic Small-Animal Imager for Vascular Visualization

Published on: July 15, 2020

Related Experiment Videos

Last Updated: Apr 27, 2026

Three-dimensional Optical-resolution Photoacoustic Microscopy

Three-dimensional Optical-resolution Photoacoustic Microscopy

Published on: May 3, 2011

Switchable Acoustic and Optical Resolution Photoacoustic Microscopy for In Vivo Small-animal Blood Vasculature Imaging

Switchable Acoustic and Optical Resolution Photoacoustic Microscopy for In Vivo Small-animal Blood Vasculature Imaging

Published on: June 26, 2017

Dual Raster-Scanning Photoacoustic Small-Animal Imager for Vascular Visualization

Dual Raster-Scanning Photoacoustic Small-Animal Imager for Vascular Visualization

Published on: July 15, 2020

Area of Science:

Biomedical Optics
Microscopy
Acoustic Imaging

Background:

Photoacoustic microscopy (PAM) offers high resolution and contrast for biological tissue imaging.
Achieving variable resolution in PAM systems is crucial for multimodal imaging applications.

Purpose of the Study:

To develop a novel multiscale photoacoustic microscope with continuously tunable lateral resolution.
To demonstrate the system's capability for imaging across a wide range of resolutions, from optical to acoustic scales.

Main Methods:

Utilized an electrical varifocal lens to control the laser spot size at the fiber bundle tip.
Employed an optical fiber bundle to deliver the tunable excitation light spot to the sample.
Developed a system capable of continuously tuning lateral resolution from approximately 1 μm to over 44.8 μm.

Main Results:

Successfully demonstrated continuously tunable lateral resolution in the photoacoustic microscope.
Achieved a resolution range spanning from sub-cellular optical scales (~1 μm) to larger acoustic scales (>44.8 μm).
Successfully imaged a mouse ear in vivo at three distinct resolutions, validating the multiscale imaging capability.

Conclusions:

The developed multiscale photoacoustic microscope provides unprecedented flexibility in imaging resolution.
This system enables multimodal imaging by combining high-resolution optical-scale details with broader acoustic-scale views.
The technology holds significant potential for various biomedical imaging applications requiring adaptable resolution.