Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Proteomics01:33

Proteomics

7.5K
A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
7.5K
Protein Kinases and Phosphatases02:54

Protein Kinases and Phosphatases

12.1K
Proteins undergo chemical modifications that trigger changes in the charge, structure, and conformation of the proteins. Phosphorylation, acetylation, glycosylation, nitrosylation, ubiquitination, lipidation, methylation, and proteolysis are various protein modifications that regulate protein activity. Such modifications are usually enzyme-driven.
Protein kinases
Many proteins in the cell are regulated by phosphorylation, the addition of a phosphate group. A family of enzymes called kinases...
12.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

From Veterinary Medicine to Illicit Drug Supply: Utilising Social Media to Explore the Rising Emergence of Veterinary Medicines in Human Health.

Brain sciences·2025
Same author

Exploring Human Misuse and Abuse of Veterinary Drugs: A Descriptive Pharmacovigilance Analysis Utilising the Food and Drug Administration's Adverse Events Reporting System (FAERS).

Toxics·2024
Same author

Exploring the Confluence of Animal Medicine and its Implications for Human Health: A Systematic Literature Review.

Current neuropharmacology·2024
Same author

The effect of 8-day oral taurine supplementation on thermoregulation during low-intensity exercise at fixed heat production in hot conditions of incremental humidity.

European journal of applied physiology·2024
Same author

Embedding retrieval practice in undergraduate biochemistry teaching using PeerWise.

Biochemistry and molecular biology education : a bimonthly publication of the International Union of Biochemistry and Molecular Biology·2023
Same author

Antimicrobial Volatiles of the Insect Pathogen <i>Metarhizium brunneum</i>.

Journal of fungi (Basel, Switzerland)·2022

Related Experiment Video

Updated: Apr 27, 2026

Quantitative Phosphoproteomics in Fatty Acid Stimulated Saccharomyces cerevisiae
15:41

Quantitative Phosphoproteomics in Fatty Acid Stimulated Saccharomyces cerevisiae

Published on: October 12, 2009

9.4K

Phosphoproteomic techniques and applications.

Ed Dudley1, A Elizabeth Bond1

  • 1Institute of Mass Spectrometry, College of Medicine, Swansea University, Swansea, United Kingdom.

Advances in Protein Chemistry and Structural Biology
|July 3, 2014
PubMed
Summary
This summary is machine-generated.

Phosphoproteomic analysis identifies protein phosphorylation sites by enriching phosphoproteins and using mass spectrometry. Advances in methods are expanding its application across biomedical fields.

Keywords:
CancerImmunityPhosphopeptidesPhosphoproteomicsStem cells

More Related Videos

Phosphopeptide Enrichment Coupled with Label-free Quantitative Mass Spectrometry to Investigate the Phosphoproteome in Prostate Cancer
12:23

Phosphopeptide Enrichment Coupled with Label-free Quantitative Mass Spectrometry to Investigate the Phosphoproteome in Prostate Cancer

Published on: August 2, 2018

13.8K
A Mass Spectrometry-Based Approach to Identify Phosphoprotein Phosphatases and their Interactors
10:17

A Mass Spectrometry-Based Approach to Identify Phosphoprotein Phosphatases and their Interactors

Published on: April 29, 2022

1.8K

Related Experiment Videos

Last Updated: Apr 27, 2026

Quantitative Phosphoproteomics in Fatty Acid Stimulated Saccharomyces cerevisiae
15:41

Quantitative Phosphoproteomics in Fatty Acid Stimulated Saccharomyces cerevisiae

Published on: October 12, 2009

9.4K
Phosphopeptide Enrichment Coupled with Label-free Quantitative Mass Spectrometry to Investigate the Phosphoproteome in Prostate Cancer
12:23

Phosphopeptide Enrichment Coupled with Label-free Quantitative Mass Spectrometry to Investigate the Phosphoproteome in Prostate Cancer

Published on: August 2, 2018

13.8K
A Mass Spectrometry-Based Approach to Identify Phosphoprotein Phosphatases and their Interactors
10:17

A Mass Spectrometry-Based Approach to Identify Phosphoprotein Phosphatases and their Interactors

Published on: April 29, 2022

1.8K

Area of Science:

  • Biochemistry and Molecular Biology
  • Proteomics

Background:

  • Protein phosphorylation is a key post-translational modification regulating cellular processes.
  • Understanding phosphorylation patterns is crucial for deciphering cell signaling and disease mechanisms.

Purpose of the Study:

  • To detail current methodologies in phosphoproteomic analysis.
  • To highlight advances in phosphoprotein and phosphopeptide enrichment and identification.
  • To discuss the expanding applications of phosphoproteomics in biomedical research.

Main Methods:

  • Enrichment of phosphoproteins or phosphopeptides.
  • Tandem mass spectrometry (MS/MS) analysis.
  • Identification of phosphosites based on characteristic fragmentation patterns.

Main Results:

  • Established protocols enable comprehensive phosphoproteomic profiling.
  • Mass spectrometry techniques effectively identify phosphorylated proteins and residues.
  • Significant advancements have been made in phosphoproteomic methodologies.

Conclusions:

  • Phosphoproteomic analysis is a powerful tool for studying cellular signaling.
  • Methodological improvements are driving wider adoption in diverse biomedical applications.
  • Continued research promises further refinement and expanded utility of phosphoproteomics.