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Updated: Apr 27, 2026
Constructing a Collagen Hydrogel for the Delivery of Stem Cell-loaded Chitosan Microspheres
Published on: June 1, 2012
Cell-microsphere constructs formed with human adipose-derived stem cells and gelatin microspheres promotes stemness,
Anjaneyulu Kodali1, Thiam Chye Lim, David Tai Leong
1Department of Chemical and Biomolecular Engineering, National University of Singapore, Singapore, 117585, Singapore.
Abstract:
Translation of stem cell therapies to clinical stage requires viable delivery vehicles that can control stem cell behavior. In this study, the suitability of gelatin microspheres (GMs) as such a tissue engineering platform for human adipose derived stem cells (ADSCs) is investigated. ADSCs on GMs, show a significant upregulation of well known pluripotent marker genes, together with the ability to differentiate into multiple cell lineages of adipocytes, osteocytes, and hepatocytes. In addition, the pro-angiogenic ability of ADSC-GMs is also enhanced compared to ADSCs on 2D culture plates. These results suggest that GMs can be a suitable culture platform for ADSCs with enhanced therapeutic potential.
