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Isolation of Human Neutrophils from Whole Blood and Buffy Coats
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Eosinophil purification from human bone marrow.

Tina W Wong1, Diane F Jelinek

  • 1Department of Immunology, Mayo Clinic, 200 1st Street SW, Rochester, MN, 55905, USA.

Methods in Molecular Biology (Clifton, N.J.)
|July 3, 2014
PubMed
Summary
This summary is machine-generated.

Researchers developed a new method to isolate eosinophils, a type of immune cell, from human bone marrow. This offers an alternative to traditional blood-based purification for studying these cells in allergic diseases and infections.

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Area of Science:

  • Immunology
  • Cell Biology

Background:

  • Eosinophils are innate immune cells crucial for host defense against parasites.
  • They play significant roles in conditions like asthma and allergic diseases.
  • Traditional methods for studying human eosinophils rely on purification from peripheral blood.

Purpose of the Study:

  • To present a novel protocol for isolating eosinophils from human bone marrow.
  • To provide an alternative source for eosinophil research.

Main Methods:

  • Development of a new purification protocol.
  • Isolation of eosinophils from human bone marrow samples.

Main Results:

  • Successful purification of eosinophils from human bone marrow.
  • Characterization of the newly isolated cells (details not provided in abstract).

Conclusions:

  • The new protocol provides a viable alternative for obtaining human eosinophils.
  • This method facilitates further research into eosinophil function and associated diseases.