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A general protein purification and immobilization method on controlled porosity glass: biocatalytic applications
K Engelmark Cassimjee1, M Kadow, Y Wikmark
1Department of Organic Chemistry, Arrhenius Laboratory, Stockholm University, SE-106 91 Stockholm, Sweden. karim@organ.su.se jeb@organ.su.se.
Chemical Communications (Cambridge, England)
|July 4, 2014
Abstract:
A general combined purification and immobilization method to facilitate biocatalytic process development is presented. The support material, EziG™, is based on controlled porosity glass (CPG) or polymer-coated versions thereof (HybCPG) and binds protein affinity tags. Biocatalytic reactions in aqueous and organic media with seven enzymes of biocatalytic interest are shown.
